{"id":"W2075921786","doi":"10.3791/50320","title":"GST-His purification: A Two-step Affinity Purification Protocol Yielding Full-length Purified Proteins","year":2013,"lang":"en","type":"article","venue":"Journal of Visualized Experiments","topic":"Viral Infectious Diseases and Gene Expression in Insects","field":"Biochemistry, Genetics and Molecular Biology","cited_by":18,"is_retracted":false,"has_abstract":true,"ca_institutions":"Hôtel-Dieu de Québec","funders":"Canadian Institutes of Health Research","keywords":"FLAG-tag; Tandem affinity purification; Cleave; Protein purification; Biochemistry; Protein tag; Affinity chromatography; Myc-tag; Fast protein liquid chromatography; Recombinant DNA; Sepharose; Chemistry; Nucleic acid; Nuclease; Chromatography; Fusion protein; Enzyme; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001066199,0.002142627,0.002018113,0.001425514,0.001061114,0.0008842196,0.001671487,0.001164473,0.003787053],"category_scores_gemma":[0.000630958,0.0008640782,0.001126512,0.002213637,0.0005313944,0.0009306468,0.001273098,0.003097792,0.008586025],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0007195848,"about_ca_system_score_gemma":0.0008907222,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0008195353,"about_ca_topic_score_gemma":0.001370806,"domain_scores_codex":[0.9984883,0.0002669082,0.0001770601,0.0002662422,0.0006230727,0.0001782984],"domain_scores_gemma":[0.9997184,0.00004569672,0.00003960379,0.00006260642,0.000092072,0.00004156911],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"not_applicable","study_design_scores_codex":[0.0002342434,0.00015551,0.000357857,0.001638155,0.0001190984,0.0002800859,0.0001372333,0.0002761786,0.9673551,0.0009118429,0.008972464,0.01956228],"study_design_scores_gemma":[0.00007724734,0.0002785437,0.001888272,0.00006386855,0.0001295086,0.001554715,0.00007126566,0.002633122,0.8193474,0.0008067133,0.1730595,0.00008975474],"study_design_candidate":"not_applicable","study_design_consensus":null,"genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.2038775,0.04706749,0.6851336,0.003931094,0.002415366,0.003961412,0.02424506,0.01255167,0.0168169],"genre_scores_gemma":[0.2631193,0.04465545,0.5356782,0.001676985,0.000646043,0.003424027,0.09502084,0.002161612,0.05361757],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.003787053,"threshold_uncertainty_score":0.01266891,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02744857697071437,"score_gpt":0.3978673463432288,"score_spread":0.3704187693725145,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}