{"id":"W2075921786","doi":"10.3791/50320","title":"GST-His purification: A Two-step Affinity Purification Protocol Yielding Full-length Purified Proteins","year":2013,"lang":"en","type":"article","venue":"Journal of Visualized Experiments","topic":"Viral Infectious Diseases and Gene Expression in Insects","field":"Biochemistry, Genetics and Molecular Biology","cited_by":18,"is_retracted":false,"has_abstract":true,"ca_institutions":"Hôtel-Dieu de Québec","funders":"Canadian Institutes of Health Research","keywords":"FLAG-tag; Tandem affinity purification; Cleave; Protein purification; Biochemistry; Protein tag; Affinity chromatography; Myc-tag; Fast protein liquid chromatography; Recombinant DNA; Sepharose; Chemistry; Nucleic acid; Nuclease; Chromatography; Fusion protein; Enzyme; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003215379,0.0002660292,0.0002947174,0.0001296143,0.0002195788,0.0001904244,0.0003688629,0.0001830789,0.0006503948],"category_scores_gemma":[0.0001903461,0.0002239474,0.0002644807,0.0001546163,0.00006544396,0.00005541293,0.00009827282,0.0001927403,0.00005993037],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00005868149,"about_ca_system_score_gemma":0.0001618263,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00004029835,"about_ca_topic_score_gemma":0.000001385712,"domain_scores_codex":[0.9978797,0.0002336455,0.0008128984,0.0003382773,0.0004232347,0.0003122285],"domain_scores_gemma":[0.9978845,0.00001545684,0.0008688283,0.0004518204,0.0005103896,0.0002690168],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0005142438,0.0007009652,0.0003142493,0.00003532811,0.0001149048,0.000003328431,0.0001358283,0.00002769336,0.9830311,0.0001012186,0.01321819,0.001802937],"study_design_scores_gemma":[0.003416721,0.001255476,0.0005451101,0.00008964038,0.00002636314,0.00005522141,0.0002602897,0.00009916551,0.9576358,0.0001422346,0.03617598,0.0002979559],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9631083,0.0004696646,0.008307447,0.0002901335,0.0006141331,0.02467167,0.000009513658,0.00004146342,0.002487673],"genre_scores_gemma":[0.9679697,0.00004432391,0.006849506,0.0002834125,0.00088548,0.02337086,0.00002383452,0.00005002366,0.0005228626],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.02539528,"threshold_uncertainty_score":0.913231,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02744857697071437,"score_gpt":0.3978673463432288,"score_spread":0.3704187693725145,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}