{"id":"W2076251130","doi":"10.1042/bj3510833","title":"Identification of Glu-519 as the catalytic nucleophile in β-mannosidase 2A from Cellulomonas fimi","year":2000,"lang":"en","type":"article","venue":"Biochemical Journal","topic":"Studies on Chitinases and Chitosanases","field":"Biochemistry, Genetics and Molecular Biology","cited_by":28,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of British Columbia","funders":"","keywords":"Chemistry; Nucleophile; Tripeptide; Glycosyl; Covalent bond; Glycoside hydrolase; Stereochemistry; Electrospray ionization; Residue (chemistry); Peptide; Active site; Hydrolysis; Enzyme; Chromatography; Mass spectrometry; Catalysis; Biochemistry; Organic chemistry","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001083818,0.0003416973,0.0001839602,0.0001090434,0.0001475176,0.0003045317,0.000195123,0.000332899,0.0005809153],"category_scores_gemma":[0.0002345208,0.000136513,0.0002531723,0.0001357779,0.0001618406,0.0001734843,0.000140369,0.0002304784,0.0004043946],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003499016,"about_ca_system_score_gemma":0.0001929484,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001358801,"about_ca_topic_score_gemma":0.0007804185,"domain_scores_codex":[0.9999332,0.00001038134,0.000004549274,0.0000203263,0.00001710481,0.0000144685],"domain_scores_gemma":[0.9999245,0.00001222429,0.00002757223,0.000007815271,0.00001076464,0.00001711514],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001160235,0.000008636395,0.000758813,0.00001992192,0.000005030676,0.0001364662,0.00002326798,0.000085088,0.9972703,0.00005013494,0.00001740047,0.001508918],"study_design_scores_gemma":[0.000009123517,0.0001585606,0.01834938,0.000006682117,0.00001443829,0.0005410208,0.00003415928,0.0009443146,0.9784901,0.00004957317,0.001396411,0.000006257287],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9965141,0.0004834511,0.002166958,0.00003767641,0.000009297212,0.00001324201,0.0001923542,0.00002572419,0.0005571711],"genre_scores_gemma":[0.9939908,0.0002783985,0.003558168,0.00004644354,0.00000519409,0.00001816684,0.0008601759,0.00001525585,0.001227371],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001358801,"threshold_uncertainty_score":0.002701819,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.005985851497258387,"score_gpt":0.2356385467441231,"score_spread":0.2296526952468647,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}