{"id":"W2077577750","doi":"10.1186/1472-6750-11-24","title":"Modified gateway system for double shRNA expression and Cre/lox based gene expression","year":2011,"lang":"en","type":"article","venue":"BMC Biotechnology","topic":"Virus-based gene therapy research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":14,"is_retracted":false,"has_abstract":true,"ca_institutions":"Princess Margaret Cancer Centre; Ontario Institute for Cancer Research; University of Toronto; Canada Research Chairs; University Health Network","funders":"Canadian Institutes of Health Research; University of Pennsylvania","keywords":"Small hairpin RNA; Biology; Expression vector; Gene; RNA interference; Complementary DNA; Gene silencing; Green fluorescent protein; Molecular biology; Multiple cloning site; Expression cloning; Computational biology; Viral vector; cDNA library; Genetics; RNA; Recombinant DNA","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003264248,0.0002469406,0.0002477985,0.0001732473,0.0001773952,0.00001782388,0.0005632199,0.000900687,0.0000233865],"category_scores_gemma":[0.00005497322,0.000215318,0.00009537923,0.0001118439,0.0002182558,0.000005532033,0.0003462822,0.0001650305,0.00001001922],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00002728867,"about_ca_system_score_gemma":0.0001022398,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00002077626,"about_ca_topic_score_gemma":0.00003449378,"domain_scores_codex":[0.9983406,0.00008342858,0.0002639087,0.0006955173,0.0001350959,0.0004814537],"domain_scores_gemma":[0.9985673,0.00001951827,0.0001142437,0.001095197,0.00008724401,0.000116453],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.005577206,0.00007617477,0.0002229512,0.000107532,0.00001727443,0.000003099242,0.00001776169,0.00003164289,0.9927854,0.0001503022,0.0003546417,0.0006560162],"study_design_scores_gemma":[0.003052821,0.002155387,0.00005589855,0.00004350923,0.00001213895,0.00001514529,0.00007901667,0.0005962573,0.9923701,0.0001087069,0.001255997,0.0002550229],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9281638,0.001282759,0.06901149,0.0001181048,0.000142332,0.0008282838,0.00004161161,0.0001520955,0.0002595403],"genre_scores_gemma":[0.9574258,0.00009359812,0.04168801,0.00006063913,0.00009930841,0.0003115009,0.00008396229,0.00005644144,0.0001807443],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.02926201,"threshold_uncertainty_score":0.8780412,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.04751059048423877,"score_gpt":0.2828754348417885,"score_spread":0.2353648443575497,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}