{"id":"W2077757749","doi":"10.1016/j.febslet.2006.08.042","title":"Enzyme conformational dynamics during catalysis and in the ‘resting state’ monitored by hydrogen/deuterium exchange mass spectrometry","year":2006,"lang":"en","type":"article","venue":"FEBS Letters","topic":"Mass Spectrometry Techniques and Applications","field":"Chemistry","cited_by":23,"is_retracted":false,"has_abstract":true,"ca_institutions":"Western University","funders":"","keywords":"Hydrogen–deuterium exchange; Chemistry; Kinetics; Deuterium; Substrate (aquarium); Mass spectrometry; Enzyme; Enzyme kinetics; Enzyme catalysis; Carboxypeptidase A; Steady state (chemistry); Amide; Carboxypeptidase; Chromatography; Active site; Biochemistry; Physical chemistry","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001247586,0.000160878,0.0001438571,0.0001246993,0.0001415851,0.0001044632,0.0002387142,0.00005618682,0.0001444349],"category_scores_gemma":[0.000007511029,0.0001456431,0.00004827932,0.0003317439,0.00006006892,0.0001303105,0.00004857652,0.0002204826,0.000004386985],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0001844471,"about_ca_system_score_gemma":0.000006197418,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0003918845,"about_ca_topic_score_gemma":0.00003434658,"domain_scores_codex":[0.9989514,0.0000153664,0.0002743933,0.0002239341,0.0002341796,0.0003007635],"domain_scores_gemma":[0.9994932,0.00006248878,0.0001229981,0.0002742467,0.00001273865,0.0000343044],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0000135768,0.00006646189,0.02828691,0.0001478993,0.00002907233,0.00002342679,0.0001788326,0.0001110958,0.9672311,0.0004302597,0.003334231,0.000147166],"study_design_scores_gemma":[0.001782514,0.00003127725,0.0508268,0.000104037,0.00009662081,0.0002088138,0.0008492774,0.01778021,0.9164581,0.002493268,0.00798836,0.00138074],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9919459,0.000110034,0.001393793,0.002532162,0.00001027667,0.0001059089,0.0001559431,0.00009900807,0.003646929],"genre_scores_gemma":[0.9973633,0.00002198609,0.001436343,0.0002472261,0.0001197999,0.0001013822,0.0004584807,0.00001861126,0.0002328771],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.05077299,"threshold_uncertainty_score":0.5939155,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.004608405726289193,"score_gpt":0.2061328141761749,"score_spread":0.2015244084498856,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}