{"id":"W2078429697","doi":"10.1021/bi700817g","title":"Carboxyl-Terminal Disulfide Bond of Acid Sphingomyelinase Is Critical for Its Secretion and Enzymatic Function","year":2007,"lang":"en","type":"article","venue":"Biochemistry","topic":"Lysosomal Storage Disorders Research","field":"Medicine","cited_by":17,"is_retracted":false,"has_abstract":true,"ca_institutions":"McGill University; Royal Victoria Hospital","funders":"","keywords":"Chinese hamster ovary cell; Endoplasmic reticulum; Mutant; Acid sphingomyelinase; Biochemistry; Sphingomyelin; Biology; Transfection; Wild type; Endoplasmic-reticulum-associated protein degradation; Ceramide; Secretion; Molecular biology; Mutant protein; Sphingomyelin phosphodiesterase; Cell biology; Gene; Unfolded protein response; Cholesterol","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001707451,0.0003080539,0.0001950334,0.0000886234,0.0001253443,0.0003472207,0.0001503283,0.0003018545,0.001013117],"category_scores_gemma":[0.0002073951,0.0001216617,0.0002111297,0.0001189636,0.0001856768,0.0002199567,0.0001851846,0.0002604977,0.0009179885],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002493012,"about_ca_system_score_gemma":0.0002205103,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0004382567,"about_ca_topic_score_gemma":0.0002188703,"domain_scores_codex":[0.999853,0.0000222579,0.00002428461,0.00003698827,0.00004671999,0.00001668466],"domain_scores_gemma":[0.9998587,0.00002265147,0.00004576962,0.00001520617,0.00002496047,0.00003272495],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00005373427,0.000007404075,0.0002868834,0.00001914298,0.00000210546,0.00004969981,0.000006829155,0.00002872005,0.998651,0.00007851144,0.00001343531,0.0008026046],"study_design_scores_gemma":[0.00001517696,0.0000837383,0.005214543,0.00000529773,0.000008380017,0.0006855257,0.0000196627,0.0004803647,0.9903665,0.00008243943,0.003034937,0.000003442223],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9941911,0.001434581,0.002957664,0.00009303448,0.00002279426,0.00001362788,0.0002475819,0.00003905695,0.001000553],"genre_scores_gemma":[0.9953701,0.0005745501,0.002228256,0.00002677206,0.000009910209,0.000009601521,0.00073444,0.0000122659,0.001034118],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001013117,"threshold_uncertainty_score":0.003389239,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02125968073773312,"score_gpt":0.3308055836015704,"score_spread":0.3095459028638373,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}