{"id":"W2078605274","doi":"10.1074/jbc.m113.455436","title":"Structural Explanation for Allolactose (lac Operon Inducer) Synthesis by lacZ β-Galactosidase and the Evolutionary Relationship between Allolactose Synthesis and the lac Repressor","year":2013,"lang":"en","type":"article","venue":"Journal of Biological Chemistry","topic":"Enzyme Structure and Function","field":"Materials Science","cited_by":52,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Calgary","funders":"","keywords":"Lac repressor; lac operon; Operon; Beta-galactosidase; Biochemistry; Inducer; Chemistry; Active site; Repressor; Binding site; Galactosidases; Stereochemistry; Phosphofructokinase 2; Galactose; Enzyme; Biology; Mutant; Escherichia coli; Gene; Gene expression","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.00007466067,0.0003878314,0.0001707213,0.0001762751,0.0003038114,0.0003590069,0.0007385049,0.0005453744,0.00119897],"category_scores_gemma":[0.000171112,0.0001786084,0.0003253719,0.0002371742,0.0004156376,0.0005355778,0.0002535305,0.0007930544,0.0004690943],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0006343316,"about_ca_system_score_gemma":0.000288798,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0008004248,"about_ca_topic_score_gemma":0.0004608622,"domain_scores_codex":[0.9999349,0.000007972702,0.000003049895,0.00002037416,0.00001927493,0.00001431341],"domain_scores_gemma":[0.9999211,0.000007903755,0.00003228848,0.000007760076,0.00001094119,0.00001995121],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002312038,0.00008725189,0.001556705,0.0001730239,0.000009024982,0.0007363016,0.0001072846,0.002293293,0.9790536,0.01076302,0.0001990376,0.004790135],"study_design_scores_gemma":[0.00009403831,0.0009451731,0.01700647,0.00005991101,0.00007213186,0.002947348,0.0004519009,0.03267288,0.9164852,0.0112643,0.01795128,0.00004930468],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9646749,0.003678101,0.02241556,0.0008620832,0.0000787726,0.00004065511,0.0002783778,0.0002230706,0.007748294],"genre_scores_gemma":[0.987155,0.001642887,0.007908019,0.0001724823,0.00002810575,0.00003437774,0.0005971292,0.00003049753,0.002431495],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.00119897,"threshold_uncertainty_score":0.004602373,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03078810025960425,"score_gpt":0.2475144548833668,"score_spread":0.2167263546237625,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}