{"id":"W2078704504","doi":"10.1046/j.1365-2958.2000.01836.x","title":"Localization of the histidine kinase PilS to the poles of <i>Pseudomonas aeruginosa</i> and identification of a localization domain","year":2000,"lang":"en","type":"article","venue":"Molecular Microbiology","topic":"Bacterial Genetics and Biotechnology","field":"Biochemistry, Genetics and Molecular Biology","cited_by":49,"is_retracted":false,"has_abstract":true,"ca_institutions":"Calgary General Hospital; University of Calgary","funders":"University of Calgary","keywords":"Biology; Histidine kinase; Transmembrane domain; Transmembrane protein; Subcellular localization; Green fluorescent protein; Linker; PAS domain; Cell biology; Histidine; Response regulator; Fusion protein; Biochemistry; Two-component regulatory system; Pseudomonas aeruginosa; Protein kinase domain; Cytoplasm; Membrane; Gene; Transcription factor; Genetics; Amino acid; Bacteria; Mutant","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001727825,0.000113813,0.0001715877,0.00004203749,0.00004543212,0.000004108284,0.0002600453,0.0002097509,0.00001296913],"category_scores_gemma":[0.00004317333,0.0000784558,0.00006433959,0.0001999894,0.0003652182,0.00000139157,0.0001209795,0.00004406457,0.000001212534],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000006176676,"about_ca_system_score_gemma":0.00003166476,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0000402659,"about_ca_topic_score_gemma":0.00003869621,"domain_scores_codex":[0.9990633,0.0001433446,0.000383605,0.0002361077,0.00004358827,0.0001300447],"domain_scores_gemma":[0.999224,0.000006609876,0.0001993694,0.0004352642,0.0001140448,0.00002068066],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00007375158,0.00004705949,0.0003548165,0.00002467663,0.0000288333,2.67521e-7,0.00006081888,0.0006763194,0.9957164,0.0003750819,0.0002020027,0.002439956],"study_design_scores_gemma":[0.0002527012,0.0001950877,0.0003405705,0.00001548086,0.00002658461,0.00001797539,0.00002394753,0.00004201404,0.9694688,0.0001325385,0.02940634,0.00007796098],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9787794,0.0009618185,0.01913038,0.0006025067,0.0000796902,0.0003135151,0.00009582495,0.000003478214,0.000033351],"genre_scores_gemma":[0.999034,0.0002042695,0.0002541168,0.0003044672,0.00002010741,0.000008611415,0.0001129598,0.00001142437,0.00004999432],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.02920434,"threshold_uncertainty_score":0.3199335,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.002885919849650763,"score_gpt":0.1917115332380797,"score_spread":0.188825613388429,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}