{"id":"W2078704504","doi":"10.1046/j.1365-2958.2000.01836.x","title":"Localization of the histidine kinase PilS to the poles of <i>Pseudomonas aeruginosa</i> and identification of a localization domain","year":2000,"lang":"en","type":"article","venue":"Molecular Microbiology","topic":"Bacterial Genetics and Biotechnology","field":"Biochemistry, Genetics and Molecular Biology","cited_by":49,"is_retracted":false,"has_abstract":true,"ca_institutions":"Calgary General Hospital; University of Calgary","funders":"University of Calgary","keywords":"Biology; Histidine kinase; Transmembrane domain; Transmembrane protein; Subcellular localization; Green fluorescent protein; Linker; PAS domain; Cell biology; Histidine; Response regulator; Fusion protein; Biochemistry; Two-component regulatory system; Pseudomonas aeruginosa; Protein kinase domain; Cytoplasm; Membrane; Gene; Transcription factor; Genetics; Amino acid; Bacteria; Mutant","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0000769619,0.0002135387,0.0001001091,0.0001838343,0.0002715254,0.0002969769,0.0001552797,0.0002064985,0.001030131],"category_scores_gemma":[0.0001394092,0.0001282593,0.0001522235,0.0001063934,0.0002707085,0.0001615862,0.000249209,0.0003985803,0.00070678],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0009648776,"about_ca_system_score_gemma":0.0003377058,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.002494799,"about_ca_topic_score_gemma":0.002964425,"domain_scores_codex":[0.9999043,0.00001156006,0.000004369163,0.00001980109,0.00003834035,0.00002159028],"domain_scores_gemma":[0.9999238,0.00001173096,0.0000169434,0.000008728,0.00001477881,0.00002396944],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00004061534,0.00000681491,0.000152771,0.00001792232,7.594178e-7,0.0000479319,0.00001296129,0.00003055323,0.9986799,0.0001960487,0.00005585425,0.0007578599],"study_design_scores_gemma":[0.00001425861,0.0001056325,0.01310696,0.00001343761,0.000006506283,0.0006233684,0.00007953983,0.001211911,0.9774354,0.000185529,0.007210033,0.000007627842],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9897609,0.001102194,0.004078302,0.0004250989,0.00003931983,0.0000241249,0.0004663078,0.0001059526,0.003997836],"genre_scores_gemma":[0.9900776,0.0005736227,0.002855618,0.00009390193,0.00001552995,0.00001917549,0.001036958,0.00004003343,0.005287521],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.002494799,"threshold_uncertainty_score":0.007000744,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.002885919849650763,"score_gpt":0.1917115332380797,"score_spread":0.188825613388429,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}