{"id":"W2078964762","doi":"10.3791/51890","title":"High-throughput Titration of Luciferase-expressing Recombinant Viruses","year":2014,"lang":"en","type":"article","venue":"Journal of Visualized Experiments","topic":"Viral Infectious Diseases and Gene Expression in Insects","field":"Biochemistry, Genetics and Molecular Biology","cited_by":14,"is_retracted":false,"has_abstract":true,"ca_institutions":"Ottawa Hospital; University of Ottawa; Ontario Institute for Cancer Research","funders":"","keywords":"Luciferase; Virus quantification; Titer; Luciferin; Microtiter plate; Titration; Plate reader; Bioluminescence; Context (archaeology); Recombinant DNA; Virology; Virus; Chemistry; Biology; Cell culture; Molecular biology; Biochemistry; Transfection","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002361476,0.0001346528,0.0002564061,0.00006774043,0.00007508813,0.00002848389,0.0001497555,0.00009848215,0.0000744189],"category_scores_gemma":[0.0001284421,0.0001099547,0.0001750413,0.00005796906,0.00004860684,0.00001824888,0.00005708021,0.00006702332,0.000003565886],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0000166145,"about_ca_system_score_gemma":0.00005701358,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0000231567,"about_ca_topic_score_gemma":8.422044e-7,"domain_scores_codex":[0.9987815,0.0001558063,0.0005329144,0.0001514417,0.0002343503,0.0001439755],"domain_scores_gemma":[0.9989141,0.00001556195,0.0005810442,0.0002025836,0.0001908255,0.00009584423],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0003676128,0.0003323479,0.001011241,0.00001667515,0.00007696536,0.000001556447,0.00008509355,0.00009295778,0.9904009,0.0001205833,0.006802248,0.0006917985],"study_design_scores_gemma":[0.001757509,0.001151254,0.0006118789,0.00009990719,0.00002526507,0.00001502438,0.00006007537,0.0000314441,0.9853478,0.0002598264,0.01051639,0.0001235953],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9911524,0.0009033818,0.006569805,0.00001757271,0.0009581582,0.00007179403,0.000003302907,0.000004980835,0.0003185983],"genre_scores_gemma":[0.9966974,0.0001722379,0.002386223,0.0002480841,0.0003755474,0.000004737993,0.00000949841,0.00001994227,0.00008638977],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.005544934,"threshold_uncertainty_score":0.4483822,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02330902502807413,"score_gpt":0.3979110964352859,"score_spread":0.3746020714072118,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}