{"id":"W2080427880","doi":"10.1021/bi301087c","title":"Activity-Based Protein Profiling of the <i>Escherichia coli</i> GlpG Rhomboid Protein Delineates the Catalytic Core","year":2012,"lang":"en","type":"article","venue":"Biochemistry","topic":"Protein Structure and Dynamics","field":"Biochemistry, Genetics and Molecular Biology","cited_by":50,"is_retracted":false,"has_abstract":true,"ca_institutions":"Steacie Institute for Molecular Sciences; University of Ottawa","funders":"","keywords":"Proteases; Rhomboid; Transmembrane domain; Protease; Chemistry; Active site; Catalytic triad; Escherichia coli; Biochemistry; Transmembrane protein; Protein structure; Lipid bilayer; Biophysics; Enzyme; Biology; Amino acid; Membrane","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002591118,0.000261015,0.0001811059,0.00001051351,0.0001650595,0.00001988158,0.0005387599,0.0002770895,0.000006049782],"category_scores_gemma":[0.0002062893,0.0001622989,0.0001739294,0.0001747232,0.0002828055,0.000006125265,0.0002043523,0.0002484898,0.000002445541],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00002849509,"about_ca_system_score_gemma":0.0002958758,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00002643214,"about_ca_topic_score_gemma":0.000004591896,"domain_scores_codex":[0.9987811,0.00005247046,0.0002419497,0.0002981018,0.0002367412,0.0003896069],"domain_scores_gemma":[0.9986507,0.00001295044,0.0002427178,0.0008830776,0.0001185401,0.00009200047],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00009798197,0.00007667683,0.002276976,0.0001579094,0.00003738915,3.010217e-7,0.00001390232,0.00001938593,0.9968677,0.00004988176,0.0001052001,0.0002967645],"study_design_scores_gemma":[0.000320907,0.00004697796,0.0005176196,0.00005398432,0.00003258009,0.000005370102,0.00003273432,0.0002570167,0.9975723,0.0000386033,0.0009055695,0.000216347],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9965379,0.0008105345,0.0008980057,0.0002458737,0.00008375155,0.0009171846,0.00007260658,0.00001846893,0.000415716],"genre_scores_gemma":[0.9968837,0.000003091376,0.001673284,0.0002030019,0.000350793,0.0002004113,0.00008926255,0.00003442161,0.0005620453],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001759356,"threshold_uncertainty_score":0.6618358,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01003744926759276,"score_gpt":0.2340370146048209,"score_spread":0.2239995653372282,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}