{"id":"W2080832714","doi":"10.1038/nsb950","title":"Opioid receptor random mutagenesis reveals a mechanism for G protein–coupled receptor activation","year":2003,"lang":"en","type":"article","venue":"Nature Structural & Molecular Biology","topic":"Receptor Mechanisms and Signaling","field":"Biochemistry, Genetics and Molecular Biology","cited_by":93,"is_retracted":false,"has_abstract":false,"ca_institutions":"AstraZeneca (Canada)","funders":"National Institute on Drug Abuse; National Institutes of Health; Centre National de la Recherche Scientifique; Institut National de la Santé et de la Recherche Médicale","keywords":"G protein-coupled receptor; Rhodopsin; Mutagenesis; Receptor; G protein; Opioid receptor; 5-HT5A receptor; Point mutation; Chemistry; 5-HT7 receptor; Biophysics; Cell biology; Mutant; Biology; Biochemistry; Opioid; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["metaepi_narrow","research_integrity"],"consensus_categories":[],"category_scores_codex":[0.0004194398,0.0004855029,0.0004861487,0.0001128374,0.0002581189,0.0000475989,0.000359465,0.001396144,0.0003201043],"category_scores_gemma":[0.001048415,0.0004171448,0.0003806337,0.000198669,0.00007633946,0.00001128167,0.00007082237,0.0003875423,0.000008535688],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00005181817,"about_ca_system_score_gemma":0.0001227565,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0000105455,"about_ca_topic_score_gemma":0.000002449447,"domain_scores_codex":[0.9974059,0.0003400636,0.0004683472,0.0009560674,0.0001879032,0.000641692],"domain_scores_gemma":[0.9985786,0.0000278523,0.0002928189,0.0005082928,0.0004306793,0.0001617265],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0004390059,0.00001372438,0.0000143397,0.00003494092,0.0001487259,6.874624e-7,0.00002173256,0.00001226128,0.9365355,0.0617453,0.0002924202,0.0007413998],"study_design_scores_gemma":[0.002372416,0.0005799952,0.000007832592,0.0000266348,0.00003594713,0.00001114965,0.00004015768,0.00006326663,0.9616282,0.02389298,0.0108301,0.0005113218],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8673183,0.0007873726,0.1285772,0.0002749281,0.001006877,0.001797224,0.00009092856,0.0000488121,0.00009837976],"genre_scores_gemma":[0.9549507,0.00004320322,0.04144482,0.00114646,0.0004022723,0.0003808829,0.001078445,0.00008601186,0.0004672426],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.08763237,"threshold_uncertainty_score":0.9999003,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.005147854237649091,"score_gpt":0.2651194816219914,"score_spread":0.2599716273843423,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}