{"id":"W2080832816","doi":"10.1016/j.bbrc.2005.10.157","title":"Role of acidic amino acid residues in chitooligosaccharide-binding to Streptomyces sp. N174 chitosanase","year":2005,"lang":"en","type":"article","venue":"Biochemical and Biophysical Research Communications","topic":"Studies on Chitinases and Chitosanases","field":"Biochemistry, Genetics and Molecular Biology","cited_by":29,"is_retracted":false,"has_abstract":false,"ca_institutions":"Université de Sherbrooke","funders":"Natural Sciences and Engineering Research Council of Canada; Ministry of Education, Culture, Sports, Science and Technology","keywords":"Oligosaccharide; Binding site; Enzyme; Chitosanase; Chemistry; Tryptophan; Biochemistry; Quenching (fluorescence); Residue (chemistry); Stereochemistry; Fluorescence; Amino acid","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002379499,0.0001697586,0.0002371708,0.0001428127,0.0002119461,0.00003017242,0.000778211,0.0001211731,0.00001164957],"category_scores_gemma":[0.000500979,0.0001512305,0.00009023193,0.0004675986,0.0007773916,0.000009342978,0.0009152709,0.0003048207,0.00001419979],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001958386,"about_ca_system_score_gemma":0.00006746712,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0001763553,"about_ca_topic_score_gemma":0.0004600368,"domain_scores_codex":[0.9983636,0.0001419544,0.0003265399,0.0004105038,0.0003268806,0.000430537],"domain_scores_gemma":[0.9985033,0.0001427844,0.00005509541,0.000951123,0.0001241527,0.0002235774],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00009105048,0.0003903323,0.003303518,0.00001896349,0.0000266848,4.568897e-7,0.0001157387,0.000001196282,0.9879503,0.0006147461,0.0005607136,0.006926263],"study_design_scores_gemma":[0.0002808419,0.0002515231,0.01510163,0.00006649896,0.000008222617,0.000002168074,0.001174247,0.00004589338,0.9800212,0.00022166,0.002652521,0.0001735555],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9926972,0.003185726,0.0000024828,0.002691895,0.000009291756,0.0002468679,0.0001063138,0.00001129693,0.001048961],"genre_scores_gemma":[0.9967735,0.001727103,0.0009331934,0.00008206159,0.0002221691,0.00008960504,0.0001236369,0.00001558139,0.00003308904],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.01179811,"threshold_uncertainty_score":0.6166999,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03727260110337503,"score_gpt":0.3534349044154687,"score_spread":0.3161623033120937,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}