{"id":"W2081391589","doi":"10.1016/j.pep.2011.11.013","title":"Thermolabile antifreeze protein produced in Escherichia coli for structural analysis","year":2011,"lang":"en","type":"article","venue":"Protein Expression and Purification","topic":"Physiological and biochemical adaptations","field":"Environmental Science","cited_by":9,"is_retracted":false,"has_abstract":false,"ca_institutions":"Memorial University of Newfoundland; Queen's University","funders":"Canadian Institutes of Health Research; Natural Sciences and Engineering Research Council of Canada; Canada Research Chairs","keywords":"Antifreeze protein; Thermolabile; Escherichia coli; Recombinant DNA; Affinity chromatography; Biochemistry; Chemistry; Antibody; Biology; Antifreeze; Molecular biology; Gene; Enzyme","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.000130744,0.00009437725,0.00011626,0.00003004289,0.00009343516,0.00001353786,0.0001220174,0.00006771596,0.0004676442],"category_scores_gemma":[0.00006390426,0.00006417288,0.00004101373,0.0003303082,0.00008337889,0.0001212975,0.00004940007,0.00006938778,0.00001179773],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001796083,"about_ca_system_score_gemma":0.000003944323,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0001026771,"about_ca_topic_score_gemma":0.00001190868,"domain_scores_codex":[0.9991918,0.00005532636,0.0001772929,0.0003337948,0.00009879914,0.0001429904],"domain_scores_gemma":[0.9996657,0.00001185277,0.00008576138,0.0001663082,0.00001172476,0.00005867465],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0000681538,0.00007023678,0.003062997,0.000008850081,0.00000498306,1.022461e-7,0.0002213987,0.000006923782,0.9936963,0.000157539,0.00001699295,0.002685529],"study_design_scores_gemma":[0.0001664967,0.00004557937,0.1396921,0.00001045413,0.00001087976,6.045867e-8,0.00002960749,0.001426467,0.8522195,0.006134104,0.000158689,0.0001060961],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9974754,0.00003048721,0.0007369367,0.0001076769,0.000007789376,0.001191022,0.000008974095,0.00002686878,0.0004148343],"genre_scores_gemma":[0.9871376,0.000002130059,0.01184305,0.00002518865,0.00001259295,0.0007294999,0.00003014971,0.000004640016,0.0002151685],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.1414769,"threshold_uncertainty_score":0.5120376,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02866731558062132,"score_gpt":0.2381800144306835,"score_spread":0.2095126988500621,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}