{"id":"W2081391589","doi":"10.1016/j.pep.2011.11.013","title":"Thermolabile antifreeze protein produced in Escherichia coli for structural analysis","year":2011,"lang":"en","type":"article","venue":"Protein Expression and Purification","topic":"Physiological and biochemical adaptations","field":"Environmental Science","cited_by":9,"is_retracted":false,"has_abstract":false,"ca_institutions":"Memorial University of Newfoundland; Queen's University","funders":"Canadian Institutes of Health Research; Natural Sciences and Engineering Research Council of Canada; Canada Research Chairs","keywords":"Antifreeze protein; Thermolabile; Escherichia coli; Recombinant DNA; Affinity chromatography; Biochemistry; Chemistry; Antibody; Biology; Antifreeze; Molecular biology; Gene; Enzyme","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003376096,0.0007120459,0.0004268627,0.0004202552,0.0003201041,0.0003824636,0.000492974,0.0004506653,0.0009553996],"category_scores_gemma":[0.0003192982,0.0002714591,0.0004275576,0.0006609248,0.0003156063,0.0002926314,0.0002864832,0.001147793,0.0006006151],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004505266,"about_ca_system_score_gemma":0.0004112421,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001880247,"about_ca_topic_score_gemma":0.002582271,"domain_scores_codex":[0.9998085,0.00002989792,0.00001792485,0.00003953464,0.00005727192,0.0000469046],"domain_scores_gemma":[0.9997248,0.00005705273,0.00005476744,0.00005525523,0.00005937175,0.00004871669],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0000726051,0.00003577488,0.00015362,0.00002380315,0.000005828854,0.00003710163,0.00002117629,0.00006516888,0.9988814,0.00008707056,0.00005225586,0.0005640846],"study_design_scores_gemma":[0.00001111057,0.00007588037,0.002196833,0.000003484537,0.00001606722,0.0001273269,0.00002313711,0.0003910782,0.9946561,0.00004469225,0.002449606,0.000004560679],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9673759,0.0006921265,0.02717376,0.000346588,0.00009674983,0.0001502968,0.002818259,0.000274218,0.00107218],"genre_scores_gemma":[0.9429953,0.00121836,0.0316623,0.000224562,0.0000393882,0.0001981426,0.01276629,0.0003128528,0.01058289],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001880247,"threshold_uncertainty_score":0.003738582,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02866731558062132,"score_gpt":0.2381800144306835,"score_spread":0.2095126988500621,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}