{"id":"W2081607456","doi":"10.1016/j.pep.2003.10.005","title":"Expression of human, mouse, and rat m-calpains in Escherichia coli and in murine fibroblasts","year":2003,"lang":"en","type":"article","venue":"Protein Expression and Purification","topic":"Calpain Protease Function and Regulation","field":"Biochemistry, Genetics and Molecular Biology","cited_by":6,"is_retracted":false,"has_abstract":false,"ca_institutions":"Queen's University","funders":"","keywords":"Calpain; Escherichia coli; Protein subunit; Molecular biology; Biology; Proteases; Sf9; Recombinant DNA; Biochemistry; Gene; Enzyme","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0005386283,0.0004945712,0.0002858756,0.0004216103,0.00032656,0.0006051199,0.0004089004,0.0003822001,0.0006192815],"category_scores_gemma":[0.000276723,0.0002743241,0.0004267661,0.0005674871,0.0002032164,0.0002673441,0.0001983042,0.0007890937,0.0005072153],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0006544745,"about_ca_system_score_gemma":0.0002993063,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001401366,"about_ca_topic_score_gemma":0.001845881,"domain_scores_codex":[0.9997062,0.00005848152,0.00004708058,0.00004819127,0.00007764511,0.00006234886],"domain_scores_gemma":[0.9997362,0.00006344088,0.00004910529,0.00006505654,0.00003927026,0.00004683618],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001924721,0.00003880018,0.0002344753,0.00006081424,0.000008500118,0.00007842825,0.00004775509,0.000107958,0.9973047,0.000531048,0.00008461318,0.001310401],"study_design_scores_gemma":[0.00002002387,0.0001100263,0.001610151,0.00001149635,0.00001894924,0.000259841,0.00003714481,0.0008051944,0.9932927,0.00008428389,0.003745136,0.000005100318],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9647948,0.005013687,0.02277337,0.0003938867,0.0001609293,0.0001794609,0.003360834,0.0001944544,0.003128722],"genre_scores_gemma":[0.9401537,0.00382606,0.03830299,0.0001394919,0.00004112647,0.0001425392,0.008082458,0.00009331192,0.009218375],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001401366,"threshold_uncertainty_score":0.004748583,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.009436084343843344,"score_gpt":0.2347145560752281,"score_spread":0.2252784717313847,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}