{"id":"W2081607456","doi":"10.1016/j.pep.2003.10.005","title":"Expression of human, mouse, and rat m-calpains in Escherichia coli and in murine fibroblasts","year":2003,"lang":"en","type":"article","venue":"Protein Expression and Purification","topic":"Calpain Protease Function and Regulation","field":"Biochemistry, Genetics and Molecular Biology","cited_by":6,"is_retracted":false,"has_abstract":false,"ca_institutions":"Queen's University","funders":"","keywords":"Calpain; Escherichia coli; Protein subunit; Molecular biology; Biology; Proteases; Sf9; Recombinant DNA; Biochemistry; Gene; Enzyme","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002814754,0.0001066028,0.0001169771,0.00008738712,0.00005658777,0.00001456876,0.00003042659,0.0001535341,0.00001441294],"category_scores_gemma":[0.00008808917,0.00009443558,0.00001387167,0.0000836656,0.00006913475,0.000012776,0.00003548019,0.00007868911,2.781768e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000006692932,"about_ca_system_score_gemma":0.00001568909,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00001199765,"about_ca_topic_score_gemma":0.00001850011,"domain_scores_codex":[0.9991584,0.0001364948,0.0002363779,0.0002864183,0.00007978226,0.000102565],"domain_scores_gemma":[0.999652,0.000008648064,0.0001006328,0.0001492229,0.00003113972,0.00005838147],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001197373,0.00007093848,0.004007262,0.0000809132,0.000001085233,2.526823e-7,0.0000834408,0.00001279627,0.9946452,0.0002167463,0.0000571284,0.0007045419],"study_design_scores_gemma":[0.0009861977,0.00009027857,0.01346148,0.0001373668,0.000001582539,0.000001659975,0.00009131279,0.0001019964,0.9822727,0.0002248262,0.002520802,0.0001097618],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9976901,0.000938068,0.0005803794,0.00006493257,0.00001603371,0.0005497515,0.000002014027,0.000006568403,0.0001520846],"genre_scores_gemma":[0.9977981,0.0002005148,0.001199324,0.00002294092,0.00001641171,0.0001050154,0.00003325416,0.00001038357,0.0006139996],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.01237242,"threshold_uncertainty_score":0.3850971,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.009436084343843344,"score_gpt":0.2347145560752281,"score_spread":0.2252784717313847,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}