{"id":"W2082280792","doi":"10.1074/jbc.m310638200","title":"Stable Gene Silencing in Human Monocytic Cell Lines Using Lentiviral-delivered Small Interference RNA","year":2004,"lang":"en","type":"article","venue":"Journal of Biological Chemistry","topic":"RNA Interference and Gene Delivery","field":"Biochemistry, Genetics and Molecular Biology","cited_by":41,"is_retracted":false,"has_abstract":true,"ca_institutions":"Vancouver Coastal Health; University of British Columbia; Vancouver Coastal Health Research Institute","funders":"Canadian Institutes of Health Research; Michael Smith Health Research BC","keywords":"Small hairpin RNA; Gene silencing; Transfection; RNA interference; Monocyte; Biology; Molecular biology; Viral vector; Cell biology; Cell culture; RNA; Gene; Immunology; Genetics; Gene knockdown","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002333647,0.0002142052,0.0003097917,0.00004342197,0.00005580882,0.00003351452,0.0004076137,0.0002845692,0.00003907411],"category_scores_gemma":[0.00007914354,0.0001707562,0.0001851408,0.00009559322,0.00008953731,0.00001158554,0.0001598053,0.0003058348,0.000002668238],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0001011999,"about_ca_system_score_gemma":0.0001318096,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00005188365,"about_ca_topic_score_gemma":0.000007169657,"domain_scores_codex":[0.9986787,0.00003761895,0.0005860521,0.000288043,0.0001023207,0.0003072377],"domain_scores_gemma":[0.999168,0.00001263609,0.0003461192,0.0001960877,0.0001725714,0.0001045415],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001297843,0.0001579587,0.001614922,0.00002765889,0.00002505379,0.00005969519,0.00003524675,0.002865544,0.9948759,7.297248e-7,0.00001967695,0.0001878205],"study_design_scores_gemma":[0.0009636852,0.0004489728,0.0005253752,0.0001216205,0.00001569307,0.00009390208,0.0002218604,0.00006538726,0.9971036,0.0001954822,0.00003122046,0.0002131999],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9961801,0.001371804,0.00190546,0.00002148492,0.00009160819,0.00005247,0.000008277217,0.000005026991,0.0003638379],"genre_scores_gemma":[0.9976158,0.0004996582,0.00133899,0.00007647005,0.0003274089,0.000001672026,0.00001855896,0.00001167698,0.0001097408],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.002800157,"threshold_uncertainty_score":0.6963238,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.04550955116313571,"score_gpt":0.2697637306656945,"score_spread":0.2242541795025588,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}