{"id":"W2082280792","doi":"10.1074/jbc.m310638200","title":"Stable Gene Silencing in Human Monocytic Cell Lines Using Lentiviral-delivered Small Interference RNA","year":2004,"lang":"en","type":"article","venue":"Journal of Biological Chemistry","topic":"RNA Interference and Gene Delivery","field":"Biochemistry, Genetics and Molecular Biology","cited_by":41,"is_retracted":false,"has_abstract":true,"ca_institutions":"Vancouver Coastal Health; University of British Columbia; Vancouver Coastal Health Research Institute","funders":"Canadian Institutes of Health Research; Michael Smith Health Research BC","keywords":"Small hairpin RNA; Gene silencing; Transfection; RNA interference; Monocyte; Biology; Molecular biology; Viral vector; Cell biology; Cell culture; RNA; Gene; Immunology; Genetics; Gene knockdown","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0005128923,0.0004134426,0.0005043631,0.0002763949,0.0003323428,0.0008654555,0.0004591995,0.0004553663,0.00127032],"category_scores_gemma":[0.0002230807,0.0002855636,0.0003783318,0.0004118306,0.0003624482,0.0001946432,0.0002587639,0.001212562,0.0009402256],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005355349,"about_ca_system_score_gemma":0.0005089539,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001047594,"about_ca_topic_score_gemma":0.001879254,"domain_scores_codex":[0.9995888,0.00005121267,0.00006678385,0.00008930362,0.0001397393,0.00006420139],"domain_scores_gemma":[0.9998301,0.00003875983,0.00004470851,0.00003432313,0.00003033079,0.00002182523],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0000609025,0.00003967857,0.0001426959,0.0000834272,0.00001163505,0.00007881632,0.00005761961,0.00009987499,0.9960136,0.000561164,0.0003270746,0.002523483],"study_design_scores_gemma":[0.00005611036,0.0002405495,0.001335497,0.00001517805,0.00003895286,0.0002196866,0.00004448565,0.002141524,0.9771527,0.0001194778,0.01862535,0.00001047804],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8369582,0.008670297,0.1212486,0.0009723876,0.000826482,0.001498339,0.01468063,0.002559146,0.01258599],"genre_scores_gemma":[0.8953755,0.006399307,0.07238341,0.000638587,0.0001065081,0.001702678,0.00859953,0.0002514308,0.01454312],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.00127032,"threshold_uncertainty_score":0.004249632,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.04550955116313571,"score_gpt":0.2697637306656945,"score_spread":0.2242541795025588,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}