{"id":"W2082862406","doi":"10.1006/prep.2001.1583","title":"Design, Production, and Characterization of an Engineered Biotin Ligase (BirA) and Its Application for Affinity Purification of Staphylokinase Produced from Bacillus subtilis via Secretion","year":2002,"lang":"en","type":"article","venue":"Protein Expression and Purification","topic":"Biotin and Related Studies","field":"Biochemistry, Genetics and Molecular Biology","cited_by":10,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Calgary","funders":"","keywords":"Biotinylation; Staphylokinase; Bacillus subtilis; DNA ligase; Nattokinase; Biochemistry; Escherichia coli; Biotin; Biology; Recombinant DNA; Enzyme; Fermentation; Bacteria","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0005517072,0.0003825407,0.0005111743,0.0002969987,0.0003004487,0.0007806777,0.0004939839,0.0004020941,0.000534674],"category_scores_gemma":[0.0006111282,0.0003467584,0.0003618519,0.000396957,0.0003648277,0.000347009,0.00031207,0.0009056514,0.00060816],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.001139957,"about_ca_system_score_gemma":0.0006753274,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.003042017,"about_ca_topic_score_gemma":0.002093394,"domain_scores_codex":[0.9996486,0.00006136435,0.00004611722,0.00005617705,0.0001297127,0.00005807888],"domain_scores_gemma":[0.9996302,0.00006474069,0.00007616389,0.00003877112,0.00007683428,0.0001132784],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00008895873,0.0000474648,0.0002594105,0.00003937474,0.000005899857,0.00003207576,0.00002514565,0.0001702706,0.9976285,0.0001704429,0.00003872331,0.001493795],"study_design_scores_gemma":[0.00001124326,0.00006559079,0.001027885,0.0000024155,0.00001049648,0.0001317771,0.00001599884,0.0008691071,0.995996,0.00002945138,0.001834091,0.000006025527],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9431659,0.001789509,0.05149208,0.0005480355,0.00006571761,0.0001866671,0.001186202,0.0003135625,0.001252404],"genre_scores_gemma":[0.9543662,0.0009062444,0.03841851,0.00009976634,0.00001439866,0.00007474919,0.002960949,0.0001161383,0.003042945],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.003042017,"threshold_uncertainty_score":0.008270979,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01614937830813527,"score_gpt":0.2175807122149662,"score_spread":0.201431333906831,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}