{"id":"W2082862406","doi":"10.1006/prep.2001.1583","title":"Design, Production, and Characterization of an Engineered Biotin Ligase (BirA) and Its Application for Affinity Purification of Staphylokinase Produced from Bacillus subtilis via Secretion","year":2002,"lang":"en","type":"article","venue":"Protein Expression and Purification","topic":"Biotin and Related Studies","field":"Biochemistry, Genetics and Molecular Biology","cited_by":10,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Calgary","funders":"","keywords":"Biotinylation; Staphylokinase; Bacillus subtilis; DNA ligase; Nattokinase; Biochemistry; Escherichia coli; Biotin; Biology; Recombinant DNA; Enzyme; Fermentation; Bacteria","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002704749,0.0001404106,0.0001513106,0.000068726,0.0001205429,0.00001240725,0.00005546394,0.000203979,0.000003130813],"category_scores_gemma":[0.000152471,0.0001283142,0.00001956136,0.0001001836,0.00007060534,0.00003106631,0.00002401581,0.00005832491,3.062233e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00000512675,"about_ca_system_score_gemma":0.000009282864,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000008299516,"about_ca_topic_score_gemma":0.000001558653,"domain_scores_codex":[0.9989416,0.0001099678,0.0003066242,0.0004475315,0.00009780395,0.00009645489],"domain_scores_gemma":[0.9991132,0.00001139983,0.00031545,0.0002463631,0.0002557127,0.00005786075],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001908588,0.00014117,0.00009975407,0.000159038,0.00001570312,2.733518e-8,0.0004018052,0.000005853987,0.9838729,0.00002675675,0.000008892673,0.01507726],"study_design_scores_gemma":[0.0004123058,0.0002271046,0.004116767,0.00006417504,0.00002555658,0.000001546673,0.00005350281,0.004555393,0.9900959,0.00007188447,0.0002415492,0.0001343602],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9734147,0.001740186,0.02258001,0.0003111679,0.00003881941,0.001832436,0.00006073228,0.00001858455,0.000003322301],"genre_scores_gemma":[0.9948081,0.0013566,0.002843773,0.000006655142,0.00008190743,0.0002707509,0.0005649353,0.00001568619,0.00005155127],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0213934,"threshold_uncertainty_score":0.5232503,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01614937830813527,"score_gpt":0.2175807122149662,"score_spread":0.201431333906831,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}