{"id":"W2082908197","doi":"10.1006/abio.2000.4698","title":"A Reporter Gene Assay for High-Throughput Screening of G-Protein-Coupled Receptors Stably or Transiently Expressed in HEK293 EBNA Cells Grown in Suspension Culture","year":2000,"lang":"en","type":"article","venue":"Analytical Biochemistry","topic":"Receptor Mechanisms and Signaling","field":"Biochemistry, Genetics and Molecular Biology","cited_by":111,"is_retracted":false,"has_abstract":false,"ca_institutions":"Merck Canada Inc. (Canada); Biotechnology Research Institute","funders":"","keywords":"HEK 293 cells; Molecular biology; Biology; Forskolin; Reporter gene; Transfection; G protein-coupled receptor; Receptor; Ionomycin; Cell culture; Signal transduction; Cell biology; Biochemistry; Gene expression; Intracellular; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.002166236,0.003251805,0.004127117,0.002757207,0.001185281,0.001450799,0.003641216,0.002049747,0.004980357],"category_scores_gemma":[0.0009186775,0.001564935,0.002843403,0.002651682,0.0009946281,0.0009990999,0.001137682,0.004706029,0.005216526],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.001102492,"about_ca_system_score_gemma":0.0008515344,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001043336,"about_ca_topic_score_gemma":0.002070713,"domain_scores_codex":[0.9971825,0.0007011275,0.0002572369,0.0005473052,0.0009824188,0.0003295291],"domain_scores_gemma":[0.9987584,0.0005748303,0.00009505338,0.000224618,0.0001903075,0.0001568623],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001060578,0.0001116583,0.00006241027,0.0001020066,0.00003079856,0.00003072253,0.00001869913,0.00008584113,0.9975788,0.0001748315,0.0003029462,0.001395155],"study_design_scores_gemma":[0.00007752499,0.0002693363,0.0005485648,0.00001551739,0.00007985496,0.0001955957,0.00001251603,0.002256372,0.9934909,0.0001027104,0.002929345,0.00002175353],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.1702691,0.002169276,0.8008956,0.000630206,0.0004196044,0.002813505,0.01059613,0.006911216,0.005295436],"genre_scores_gemma":[0.2061942,0.004215811,0.7119548,0.0005705551,0.0002107874,0.007152608,0.0378792,0.001826411,0.02999551],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.004980357,"threshold_uncertainty_score":0.01666093,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01238039585136781,"score_gpt":0.2469402600033727,"score_spread":0.2345598641520049,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}