{"id":"W2083822728","doi":"10.1021/bi052370b","title":"Identification of Asp<sup>174</sup> and Asp<sup>175</sup> as the Key Catalytic Residues of Human <i>O</i>-GlcNAcase by Functional Analysis of Site-Directed Mutants","year":2006,"lang":"en","type":"article","venue":"Biochemistry","topic":"Glycosylation and Glycoproteins Research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":121,"is_retracted":false,"has_abstract":true,"ca_institutions":"Simon Fraser University","funders":"","keywords":"Chemistry; Stereochemistry; Residue (chemistry); Threonine; Enzyme; Serine; Leaving group; Mutant; Catalysis; Enzyme catalysis; Active site; Biochemistry","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004159354,0.0002224334,0.0003427084,0.0001597965,0.0001486575,0.00003495213,0.0002717294,0.0002777188,0.0001332398],"category_scores_gemma":[0.0002888613,0.0001975404,0.0002051524,0.0007685937,0.0004144144,0.0000104271,0.00013482,0.0001390311,0.000003814117],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00002546402,"about_ca_system_score_gemma":0.0001268123,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0005813413,"about_ca_topic_score_gemma":0.00006085035,"domain_scores_codex":[0.9979365,0.00009449635,0.0006997747,0.0005057065,0.0005150285,0.0002485313],"domain_scores_gemma":[0.9982635,0.00005272952,0.0003917829,0.0007226074,0.0004799989,0.00008943157],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00014874,0.0001986207,0.006976835,0.0001290095,0.0003713337,0.000002086674,0.00007180687,0.0006373014,0.9877947,0.0000172199,0.003480419,0.0001719466],"study_design_scores_gemma":[0.0005721638,0.00009451295,0.009553554,0.00002245425,0.0002741305,0.0000102353,0.0001806737,0.002606147,0.9858619,0.00003652418,0.0006067366,0.0001809464],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9966858,0.00130959,0.00008090021,0.00008881684,0.000008823965,0.0002201948,0.0006069425,0.00001562977,0.0009833087],"genre_scores_gemma":[0.9937193,0.00007070187,0.00004214335,0.00002251502,0.00007763545,0.00002480543,0.002729658,0.00002142492,0.003291838],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.002966514,"threshold_uncertainty_score":0.8055464,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.008473505556402895,"score_gpt":0.2542332618207128,"score_spread":0.2457597562643099,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}