{"id":"W2083822728","doi":"10.1021/bi052370b","title":"Identification of Asp<sup>174</sup> and Asp<sup>175</sup> as the Key Catalytic Residues of Human <i>O</i>-GlcNAcase by Functional Analysis of Site-Directed Mutants","year":2006,"lang":"en","type":"article","venue":"Biochemistry","topic":"Glycosylation and Glycoproteins Research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":121,"is_retracted":false,"has_abstract":true,"ca_institutions":"Simon Fraser University","funders":"","keywords":"Chemistry; Stereochemistry; Residue (chemistry); Threonine; Enzyme; Serine; Leaving group; Mutant; Catalysis; Enzyme catalysis; Active site; Biochemistry","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001479791,0.0005004408,0.0001976513,0.0001187059,0.00009559551,0.0002764179,0.0002825904,0.000377126,0.0007125342],"category_scores_gemma":[0.0001933634,0.0001459456,0.0003317034,0.0001685209,0.0002878497,0.0001669418,0.0001680812,0.0004005691,0.0003823447],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002874816,"about_ca_system_score_gemma":0.000198205,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000623405,"about_ca_topic_score_gemma":0.0004694665,"domain_scores_codex":[0.9998823,0.00002290689,0.00001953931,0.00002112813,0.00003455302,0.00001945685],"domain_scores_gemma":[0.9998134,0.00003046508,0.00008533834,0.00001492117,0.0000175453,0.00003836448],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0003419136,0.00003407782,0.000908586,0.00003740455,0.00001355944,0.0001793969,0.00001219751,0.0001295547,0.9967416,0.0001284511,0.00004030646,0.001432842],"study_design_scores_gemma":[0.00002763645,0.0002903489,0.008624979,0.000006055818,0.00003847989,0.001333956,0.00003260639,0.0008835364,0.9868607,0.00005185498,0.001842583,0.000007233268],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9972894,0.0005469843,0.0013804,0.00005801043,0.0000137433,0.00002228936,0.0003079481,0.00001447898,0.0003667958],"genre_scores_gemma":[0.992149,0.0004452719,0.004825875,0.00007093361,0.00001215413,0.00002283055,0.001578948,0.00002033612,0.0008746219],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0007125342,"threshold_uncertainty_score":0.002383709,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.008473505556402895,"score_gpt":0.2542332618207128,"score_spread":0.2457597562643099,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}