{"id":"W2083860129","doi":"10.3791/50031","title":"Detection of Protein Palmitoylation in Cultured Hippocampal Neurons by Immunoprecipitation and Acyl-Biotin Exchange (ABE)","year":2013,"lang":"en","type":"article","venue":"Journal of Visualized Experiments","topic":"Biotin and Related Studies","field":"Biochemistry, Genetics and Molecular Biology","cited_by":107,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of British Columbia","funders":"Canadian Institutes of Health Research","keywords":"Immunoprecipitation; Palmitoylation; Biotin; Hippocampal formation; Chemistry; Biotinylation; Cell biology; Molecular biology; Neuroscience; Biology; Biochemistry; Cysteine","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0008191672,0.00192328,0.001590516,0.001224266,0.0009067479,0.0008503213,0.001194378,0.0007643211,0.002067314],"category_scores_gemma":[0.0003506889,0.0008276985,0.000961119,0.001153362,0.0005804094,0.0007283766,0.001189994,0.002564064,0.004022285],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0007779392,"about_ca_system_score_gemma":0.0007361395,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.002305701,"about_ca_topic_score_gemma":0.008143729,"domain_scores_codex":[0.9986896,0.00008027159,0.0001559287,0.0004026914,0.000411235,0.0002602467],"domain_scores_gemma":[0.9996196,0.00006588582,0.00005708058,0.00005427005,0.0001551919,0.00004793117],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00004594346,0.00002881989,0.0001327592,0.0001130264,0.00001309293,0.00005438533,0.00004340064,0.00002858449,0.9978909,0.00008614086,0.00009290336,0.001470032],"study_design_scores_gemma":[0.00001548387,0.000111047,0.003243571,0.00002822465,0.00004517148,0.0002257947,0.00006476591,0.001188318,0.990356,0.0001487928,0.004556699,0.00001616677],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"methods","genre_scores_codex":[0.4722492,0.0195533,0.4570492,0.001123233,0.001679202,0.002112877,0.01612241,0.004349049,0.02576157],"genre_scores_gemma":[0.4187405,0.02303436,0.4985564,0.001305167,0.000454024,0.005209044,0.01770232,0.001001345,0.03399687],"genre_candidate":"methods","genre_consensus":null,"teacher_disagreement_score":0.002305701,"threshold_uncertainty_score":0.006915927,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.009829810730562208,"score_gpt":0.3316834977003623,"score_spread":0.3218536869698001,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}