{"id":"W2084590452","doi":"10.1021/ja028212e","title":"Nonequilibrium Capillary Electrophoresis of Equilibrium Mixtures − A Single Experiment Reveals Equilibrium and Kinetic Parameters of Protein−DNA Interactions","year":2002,"lang":"en","type":"article","venue":"Journal of the American Chemical Society","topic":"Microfluidic and Capillary Electrophoresis Applications","field":"Engineering","cited_by":195,"is_retracted":false,"has_abstract":true,"ca_institutions":"York University","funders":"","keywords":"Electropherogram; Chemistry; Capillary electrophoresis; Equilibrium constant; Electrophoresis; Binding constant; Macromolecule; Non-equilibrium thermodynamics; DNA; Reaction rate constant; Oligonucleotide; Thermodynamics; Kinetics; Analytical Chemistry (journal); Chromatography; Physical chemistry; Binding site; Biochemistry","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.000115633,0.0002122162,0.0005534067,0.00004433288,0.00002542695,0.00001739886,0.0003792222,0.00006086853,0.00004278147],"category_scores_gemma":[0.00005058213,0.0001622844,0.0004694534,0.000439041,0.0004846958,0.00008468996,0.000102835,0.0003236533,8.060147e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0001411109,"about_ca_system_score_gemma":0.00002210184,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00001656841,"about_ca_topic_score_gemma":9.59721e-8,"domain_scores_codex":[0.9984491,0.00005601102,0.0007102857,0.000158447,0.0003194171,0.0003067662],"domain_scores_gemma":[0.9987217,0.0001138265,0.0005833221,0.0003394654,0.000121225,0.0001204608],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003240615,0.0001247358,0.00005451376,0.00007561558,0.0002196904,6.748576e-7,0.0003827413,0.00003317975,0.9701989,0.000007315364,0.02857292,0.000297315],"study_design_scores_gemma":[0.000227421,0.000212952,0.0001101859,0.00008396835,0.00009805418,0.00007181679,0.0001665528,0.0005864574,0.9970833,0.00008822536,0.001117892,0.0001531852],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9777036,0.02133152,0.00009944961,0.0004579229,0.00005248582,0.0001973347,0.00000989725,0.00002000199,0.0001277886],"genre_scores_gemma":[0.9939622,0.004158608,0.001622992,0.00007857534,0.00008397709,0.00001350214,0.000001103734,0.00003694417,0.0000420406],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.02745503,"threshold_uncertainty_score":0.6617765,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01054809867838185,"score_gpt":0.2148924541128086,"score_spread":0.2043443554344267,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}