{"id":"W2084695909","doi":"10.1385/abab:127:2:095","title":"Evaluation of a New System for Developing Particulate Enzymes Based on the Surface (S)-Layer Protein (RsaA) of <I>Caulobacter crescentus</I>: Fusion With the b<I>-1,4-Glycanase (Cex) From the Cellulolytic Bacterium </I>Cellulomonas fimi<I> Yields a Robust, Catalytically Active Product","year":2005,"lang":"en","type":"article","venue":"Applied Biochemistry and Biotechnology","topic":"Enzyme Production and Characterization","field":"Biochemistry, Genetics and Molecular Biology","cited_by":18,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of British Columbia","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Caulobacter crescentus; Fusion protein; Chemistry; S-layer; Enzyme; Substrate (aquarium); Biochemistry; Recombinant DNA; Bacterial protein; Biology; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["metaepi_narrow"],"consensus_categories":[],"category_scores_codex":[0.001111213,0.0005929893,0.0005040048,0.00006740958,0.0004504566,0.00006165927,0.0006688147,0.0007972195,0.00003627639],"category_scores_gemma":[0.0002878206,0.0003699742,0.0001590059,0.000431301,0.0006400639,0.00002254785,0.0003441639,0.0003332505,0.000004738054],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0001428975,"about_ca_system_score_gemma":0.0005834412,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000009966241,"about_ca_topic_score_gemma":0.0001265538,"domain_scores_codex":[0.9967121,0.0001908042,0.0007112192,0.001155024,0.0006516319,0.0005792161],"domain_scores_gemma":[0.9971581,0.0001253489,0.0007205199,0.001345238,0.0005353664,0.0001153999],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00105306,0.0001771801,0.0000114648,0.0001731988,0.0002484513,0.000001157902,0.0001444807,0.0004025776,0.9921306,0.001019858,0.001043079,0.003594848],"study_design_scores_gemma":[0.001556502,0.0002842639,0.000327164,0.0002194466,0.0004136244,0.00001561707,0.0001208215,0.002266348,0.9807052,0.00004159097,0.01357161,0.0004778389],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9806396,0.001224932,0.003204746,0.01197928,0.0001005141,0.002433493,0.0001683233,0.00007747619,0.0001716403],"genre_scores_gemma":[0.9966603,0.0001477452,0.00121719,0.0002826643,0.0003903846,0.0002907958,0.0006092916,0.00006300167,0.0003386642],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.01602067,"threshold_uncertainty_score":0.9998752,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01601616426502058,"score_gpt":0.2167932839044764,"score_spread":0.2007771196394559,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}