{"id":"W2084695909","doi":"10.1385/abab:127:2:095","title":"Evaluation of a New System for Developing Particulate Enzymes Based on the Surface (S)-Layer Protein (RsaA) of <I>Caulobacter crescentus</I>: Fusion With the b<I>-1,4-Glycanase (Cex) From the Cellulolytic Bacterium </I>Cellulomonas fimi<I> Yields a Robust, Catalytically Active Product","year":2005,"lang":"en","type":"article","venue":"Applied Biochemistry and Biotechnology","topic":"Enzyme Production and Characterization","field":"Biochemistry, Genetics and Molecular Biology","cited_by":18,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of British Columbia","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Caulobacter crescentus; Fusion protein; Chemistry; S-layer; Enzyme; Substrate (aquarium); Biochemistry; Recombinant DNA; Bacterial protein; Biology; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001026354,0.001005246,0.000641812,0.0002995678,0.0002740771,0.001093623,0.0007752462,0.0007305235,0.0009554406],"category_scores_gemma":[0.0005264829,0.0003229038,0.0005807616,0.0002602894,0.0004726613,0.0007221878,0.0006127348,0.001314381,0.00113179],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0006740219,"about_ca_system_score_gemma":0.0005183835,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001323343,"about_ca_topic_score_gemma":0.001064431,"domain_scores_codex":[0.9994867,0.00007726129,0.00005042489,0.0001282285,0.0001945003,0.00006299703],"domain_scores_gemma":[0.9996291,0.00006225155,0.00008955217,0.00007142356,0.00006405231,0.00008352547],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00004312005,0.00008013559,0.00007727052,0.00002319983,0.000006892783,0.00001293415,0.00001010844,0.00005934138,0.99864,0.00005983014,0.00002726115,0.0009599672],"study_design_scores_gemma":[0.00002221444,0.0003227037,0.0003295803,0.000002469359,0.00001542438,0.00008614796,0.00001040708,0.0007609683,0.9972499,0.00001417889,0.001180387,0.000005612978],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9290635,0.001027093,0.06716117,0.0002953963,0.0001466881,0.0003771969,0.0004172896,0.000561388,0.0009503424],"genre_scores_gemma":[0.90641,0.001006251,0.08157577,0.0001344838,0.00005504216,0.0003598933,0.004135476,0.0002554464,0.006067653],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001323343,"threshold_uncertainty_score":0.005427897,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01601616426502058,"score_gpt":0.2167932839044764,"score_spread":0.2007771196394559,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}