{"id":"W2085248799","doi":"10.1074/jbc.c109.064766","title":"Real-time NMR Study of Three Small GTPases Reveals That Fluorescent 2′(3′)-O-(N-Methylanthraniloyl)-tagged Nucleotides Alter Hydrolysis and Exchange Kinetics","year":2009,"lang":"en","type":"article","venue":"Journal of Biological Chemistry","topic":"Protein Structure and Dynamics","field":"Biochemistry, Genetics and Molecular Biology","cited_by":51,"is_retracted":false,"has_abstract":true,"ca_institutions":"University Health Network; University of Toronto; Ontario Institute for Cancer Research","funders":"Canadian Institutes of Health Research","keywords":"GTPase; Nucleotide; Kinetics; Fluorescence; Chemistry; Guanine nucleotide exchange factor; Hydrolysis; Biochemistry; Biophysics; Biology; Physics; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003267259,0.0002628668,0.0005395901,0.000035753,0.00005019806,0.00002251555,0.000330168,0.0003019616,0.00005167538],"category_scores_gemma":[0.0001923073,0.0001819674,0.0001840134,0.00008236356,0.000139618,0.000005538425,0.0001270027,0.0002083533,5.525828e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0000174681,"about_ca_system_score_gemma":0.00002106502,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0000078422,"about_ca_topic_score_gemma":0.000004222705,"domain_scores_codex":[0.9986036,0.00008039056,0.0005670359,0.000306416,0.0001839664,0.0002586047],"domain_scores_gemma":[0.9987572,0.0000456095,0.0005552937,0.0003184392,0.0001604762,0.0001630049],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0004155669,0.0004168136,0.00942078,0.00003796381,0.0001184659,0.00003828207,0.00003864404,0.00001764163,0.9868655,0.000001798292,0.0001114383,0.002517107],"study_design_scores_gemma":[0.001548979,0.003851171,0.05042032,0.00006707387,0.0001374069,0.0001820658,0.0001454297,0.00002890475,0.9422942,0.0008467105,0.0001571223,0.0003205652],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9978826,0.001432488,0.0001065855,0.00009988049,0.000029827,0.0001721538,0.00002228055,0.00000833572,0.0002458555],"genre_scores_gemma":[0.9962646,0.001448474,0.001812511,0.00008556805,0.0003043197,0.000002363739,0.00002211873,0.00001253788,0.00004752905],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.04457124,"threshold_uncertainty_score":0.7420416,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01853479651131823,"score_gpt":0.2474264836822702,"score_spread":0.228891687170952,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}