{"id":"W2085799283","doi":"10.1021/bi0270642","title":"Trp-999 of β-Galactosidase (<i>Escherichia coli</i>) Is a Key Residue for Binding, Catalysis, and Synthesis of Allolactose, the Natural <i>Lac</i> Operon Inducer","year":2003,"lang":"en","type":"article","venue":"Biochemistry","topic":"Enzyme Structure and Function","field":"Materials Science","cited_by":40,"is_retracted":false,"has_abstract":true,"ca_institutions":"Concordia University; University of Calgary","funders":"","keywords":"Chemistry; Affinities; Residue (chemistry); Catalysis; Escherichia coli; Stereochemistry; Reaction rate constant; Acceptor; Enzyme; lac operon; Binding site; Kinetics; Biochemistry","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001557821,0.000639306,0.0003067281,0.0001319259,0.0001669687,0.0003910211,0.0003767666,0.0003151949,0.001127512],"category_scores_gemma":[0.0003806473,0.0001755061,0.0003355047,0.0002268305,0.000359375,0.0002961253,0.0003245704,0.000703005,0.000630851],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003198814,"about_ca_system_score_gemma":0.0001982175,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0003405563,"about_ca_topic_score_gemma":0.0002420345,"domain_scores_codex":[0.9997618,0.00006507856,0.00001591035,0.00004844778,0.00007812913,0.00003069608],"domain_scores_gemma":[0.9997569,0.00005095876,0.0001138979,0.0000242477,0.0000192907,0.00003463809],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001430187,0.00003392702,0.0004874839,0.0000572033,0.00001186479,0.0000873016,0.00001287297,0.0002341368,0.994908,0.0002259215,0.00006750598,0.003730718],"study_design_scores_gemma":[0.000008969279,0.0001597859,0.002290446,0.000008459901,0.00001672052,0.0003733698,0.00001972146,0.0006513955,0.9920711,0.00008526378,0.004305685,0.000009172405],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9769762,0.001260631,0.01824651,0.0001255216,0.00005934006,0.00006752411,0.0007062748,0.0002357922,0.00232222],"genre_scores_gemma":[0.985893,0.0006046218,0.01021793,0.00006900004,0.00001213219,0.00003720768,0.001314124,0.00009031827,0.001761758],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001127512,"threshold_uncertainty_score":0.003771901,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.007854442764096952,"score_gpt":0.22206093955676,"score_spread":0.214206496792663,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}