{"id":"W2085799283","doi":"10.1021/bi0270642","title":"Trp-999 of β-Galactosidase (<i>Escherichia coli</i>) Is a Key Residue for Binding, Catalysis, and Synthesis of Allolactose, the Natural <i>Lac</i> Operon Inducer","year":2003,"lang":"en","type":"article","venue":"Biochemistry","topic":"Enzyme Structure and Function","field":"Materials Science","cited_by":40,"is_retracted":false,"has_abstract":true,"ca_institutions":"Concordia University; University of Calgary","funders":"","keywords":"Chemistry; Affinities; Residue (chemistry); Catalysis; Escherichia coli; Stereochemistry; Reaction rate constant; Acceptor; Enzyme; lac operon; Binding site; Kinetics; Biochemistry","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003377708,0.0001806736,0.0002763996,0.00002859014,0.0001073798,0.00003368291,0.0002095439,0.0001588226,0.00009370661],"category_scores_gemma":[0.0003938768,0.0001267462,0.0000960473,0.0001692576,0.0001917115,0.000084676,0.00004091205,0.0001094136,0.000002218246],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00002489152,"about_ca_system_score_gemma":0.0001051174,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0000979871,"about_ca_topic_score_gemma":0.00001420273,"domain_scores_codex":[0.9989049,0.00004764427,0.0003125885,0.0003172975,0.0002032877,0.0002143156],"domain_scores_gemma":[0.9990155,0.0001822099,0.0002468804,0.0003849328,0.0001101419,0.00006031654],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00009715998,0.00003820909,0.0004961598,0.0001826252,0.00003073751,3.318066e-7,0.0002203298,4.065537e-7,0.9957834,0.00005262961,0.003009415,0.00008852682],"study_design_scores_gemma":[0.0003512783,0.00003687905,0.0004777982,0.00004906413,0.0001089925,0.000005144445,0.0002553741,0.000006505737,0.9972388,0.0000404126,0.001284438,0.0001452813],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9977381,0.0007153107,0.00004230428,0.0001530987,0.0001600218,0.0001857575,0.0001479508,0.00001557483,0.0008418398],"genre_scores_gemma":[0.9983385,0.00003286825,0.0008870093,0.0001284828,0.00006067625,0.00003717635,0.00001465571,0.0000174511,0.0004832254],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001724977,"threshold_uncertainty_score":0.5168559,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.007854442764096952,"score_gpt":0.22206093955676,"score_spread":0.214206496792663,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}