{"id":"W2085886437","doi":"10.1016/j.pep.2009.06.019","title":"Cloning, expression, purification, distribution and kinetics characterization of the bacterial β-galactosidase fused to the cytoplasmic transduction peptide in vitro and in vivo","year":2009,"lang":"en","type":"article","venue":"Protein Expression and Purification","topic":"RNA Interference and Gene Delivery","field":"Biochemistry, Genetics and Molecular Biology","cited_by":10,"is_retracted":false,"has_abstract":false,"ca_institutions":"Canadian Blood Services","funders":"National Natural Science Foundation of China","keywords":"Fusion protein; In vivo; In vitro; Molecular biology; Transduction (biophysics); Cytoplasm; Biology; Signal transduction; Cell biology; Biochemistry; Chemistry; Recombinant DNA; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001907121,0.0001118884,0.0001011487,0.00004543928,0.00007083078,0.00003635807,0.0000973791,0.0001139694,0.000005080267],"category_scores_gemma":[0.0000681219,0.00007749308,0.00001808623,0.0001137813,0.00004806879,0.00002311313,0.00002828926,0.00008030181,2.958097e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0000103745,"about_ca_system_score_gemma":0.00001656,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000009637759,"about_ca_topic_score_gemma":0.000011496,"domain_scores_codex":[0.9991044,0.0001380497,0.0002773003,0.0002752035,0.00009518702,0.0001098052],"domain_scores_gemma":[0.9995297,0.000006706305,0.0001371204,0.0002289942,0.00005796934,0.00003948856],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0004469269,0.00005434129,0.0005616512,0.00001402065,0.000001148527,7.198051e-8,0.0003496622,0.000006297202,0.9955614,0.00001167325,0.00003981068,0.002953053],"study_design_scores_gemma":[0.0003676185,0.00008341711,0.09533801,0.00009666167,0.000003761385,0.000002639971,0.00006027548,0.00008478727,0.901762,0.00003326968,0.002084245,0.00008331793],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9949492,0.0001209492,0.002844696,0.001305197,0.00004806594,0.0006737273,0.00004300969,0.00000465364,0.00001049561],"genre_scores_gemma":[0.9991187,0.0002718203,0.0001366246,0.00006357678,0.00006314533,0.00007561283,0.0001855531,0.000005979642,0.00007891728],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.09477636,"threshold_uncertainty_score":0.3160076,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.006825332777485335,"score_gpt":0.2167997896054332,"score_spread":0.2099744568279478,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}