{"id":"W2085886437","doi":"10.1016/j.pep.2009.06.019","title":"Cloning, expression, purification, distribution and kinetics characterization of the bacterial β-galactosidase fused to the cytoplasmic transduction peptide in vitro and in vivo","year":2009,"lang":"en","type":"article","venue":"Protein Expression and Purification","topic":"RNA Interference and Gene Delivery","field":"Biochemistry, Genetics and Molecular Biology","cited_by":10,"is_retracted":false,"has_abstract":false,"ca_institutions":"Canadian Blood Services","funders":"National Natural Science Foundation of China","keywords":"Fusion protein; In vivo; In vitro; Molecular biology; Transduction (biophysics); Cytoplasm; Biology; Signal transduction; Cell biology; Biochemistry; Chemistry; Recombinant DNA; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0005447478,0.0006081011,0.0005746915,0.0004011724,0.0004371321,0.0006643878,0.0004877646,0.000294382,0.000881117],"category_scores_gemma":[0.0004790887,0.0003934017,0.0004557603,0.0009352739,0.00045135,0.0004146297,0.0002874027,0.001515234,0.00131117],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00105061,"about_ca_system_score_gemma":0.0009644493,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.004854458,"about_ca_topic_score_gemma":0.003672922,"domain_scores_codex":[0.9996644,0.00005849453,0.00003406159,0.00005722365,0.0001009483,0.00008480335],"domain_scores_gemma":[0.9995593,0.0001218846,0.0000670902,0.00008684536,0.00009624563,0.0000685842],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001078559,0.00003655305,0.0001048678,0.00005949345,0.000004012398,0.00002243565,0.00004767152,0.0001135136,0.9977609,0.0002901074,0.0001246757,0.001327777],"study_design_scores_gemma":[0.00001002499,0.00003201888,0.001384524,0.000004550455,0.00001097549,0.0001002463,0.00001791517,0.0005473573,0.9928542,0.0001156588,0.004914009,0.000008464398],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8369362,0.006200934,0.1356701,0.001263718,0.0001562654,0.0003949056,0.01301115,0.001036604,0.005330013],"genre_scores_gemma":[0.8684052,0.006360322,0.07519158,0.0003349668,0.0000582925,0.0004002499,0.02468424,0.001004488,0.0235607],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.004854458,"threshold_uncertainty_score":0.009652436,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.006825332777485335,"score_gpt":0.2167997896054332,"score_spread":0.2099744568279478,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}