{"id":"W2086630637","doi":"10.1139/w07-007","title":"Conditions that allow for effective transfer of membrane proteins onto nitrocellulose membrane in Western blots","year":2007,"lang":"en","type":"article","venue":"Canadian Journal of Microbiology","topic":"Bacterial Genetics and Biotechnology","field":"Biochemistry, Genetics and Molecular Biology","cited_by":26,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Guelph","funders":"Natural Sciences and Engineering Research Council of Canada; Canada Research Chairs; Cystic Fibrosis Foundation","keywords":"Nitrocellulose; Blot; Membrane; Membrane protein; Lysis; Gel electrophoresis; Polyacrylamide gel electrophoresis; Sodium dodecyl sulfate; Chemistry; Chromatography; Transmembrane protein; Biochemistry; Lysis buffer; Enzyme","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":true,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.004849264,0.002164301,0.002234621,0.001584032,0.001188915,0.00196044,0.001460794,0.001471704,0.007681517],"category_scores_gemma":[0.00445879,0.001205022,0.0009382268,0.002067529,0.0009131982,0.00234659,0.0008904511,0.004016314,0.006118273],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005124533,"about_ca_system_score_gemma":0.0008075086,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0007134019,"about_ca_topic_score_gemma":0.00137354,"domain_scores_codex":[0.9956304,0.00145021,0.0007952803,0.0006874737,0.00108069,0.0003559178],"domain_scores_gemma":[0.9965097,0.001373569,0.0005463409,0.0006516427,0.0007782091,0.0001405625],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00008181754,0.0001426223,0.0001084539,0.00034746,0.0000198787,0.00008428953,0.0000769658,0.0000985501,0.995252,0.0005790376,0.000451087,0.002757658],"study_design_scores_gemma":[0.00004318137,0.0002904918,0.001937273,0.00006063576,0.00006768744,0.0006444396,0.00009293214,0.001275429,0.977684,0.0007423476,0.01712527,0.00003624803],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"empirical","genre_scores_codex":[0.1271142,0.004895767,0.8402801,0.001103802,0.001482354,0.004485978,0.004147845,0.003382257,0.01310768],"genre_scores_gemma":[0.1077861,0.01005718,0.8538812,0.00051034,0.0005668803,0.008810395,0.008071973,0.001271896,0.00904394],"genre_candidate":"empirical","genre_consensus":null,"teacher_disagreement_score":0.007681517,"threshold_uncertainty_score":0.02569723,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.00752838434940855,"score_gpt":0.2176685538384377,"score_spread":0.2101401694890292,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}