{"id":"W2086968510","doi":"10.1021/bi035667n","title":"Molecular Mechanism of the Enterococcal Aminoglycoside 6‘-<i>N</i>-Acetyltransferase‘:  Role of GNAT-Conserved Residues in the Chemistry of Antibiotic Inactivation","year":2003,"lang":"en","type":"article","venue":"Biochemistry","topic":"Biochemical and Molecular Research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":43,"is_retracted":false,"has_abstract":true,"ca_institutions":"McMaster University","funders":"","keywords":"Active site; Residue (chemistry); Biochemistry; Stereochemistry; Chemistry; Acetyltransferase; Acetyltransferases; Enterococcus hirae; Enzyme; Site-directed mutagenesis; Mutant; Aminoglycoside; Protein superfamily; Biology; Antibiotics; Acetylation; Enterococcus","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001564307,0.0004531816,0.0002817199,0.0001194887,0.0001539038,0.0003047583,0.0005506756,0.0006112925,0.0006086688],"category_scores_gemma":[0.0001149576,0.000162805,0.0003074621,0.00005500355,0.0003541413,0.0003893599,0.0002258113,0.0004068765,0.0005222781],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005526203,"about_ca_system_score_gemma":0.0002472621,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0007594734,"about_ca_topic_score_gemma":0.0003707102,"domain_scores_codex":[0.9999173,0.00001205987,0.000006430863,0.00002109946,0.00002145619,0.00002163138],"domain_scores_gemma":[0.9999421,0.000009394977,0.00001504963,0.000006979837,0.00001169655,0.00001484567],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001233973,0.00003446299,0.0005504753,0.0001103444,0.00001048869,0.0002540518,0.00002361317,0.0003894615,0.9948604,0.001249803,0.00007235657,0.002321206],"study_design_scores_gemma":[0.00001660354,0.0001522443,0.002842281,0.000008273011,0.0000121729,0.0005503099,0.00002298026,0.004117528,0.989854,0.0003096288,0.002104884,0.000009093276],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9759105,0.004887078,0.01459399,0.0004819901,0.0000870986,0.0001155721,0.0001693109,0.000277652,0.003476599],"genre_scores_gemma":[0.9908477,0.001496531,0.004297051,0.00008195946,0.00001449326,0.00002910801,0.0001644379,0.00001638905,0.003052308],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0007594734,"threshold_uncertainty_score":0.004009604,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.006619452523878033,"score_gpt":0.2251150008053462,"score_spread":0.2184955482814681,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}