{"id":"W2087014554","doi":"10.1002/bit.22278","title":"Optimization of elastin‐like polypeptide fusions for expression and purification of recombinant proteins in plants","year":2009,"lang":"en","type":"article","venue":"Biotechnology and Bioengineering","topic":"Transgenic Plants and Applications","field":"Biochemistry, Genetics and Molecular Biology","cited_by":136,"is_retracted":false,"has_abstract":true,"ca_institutions":"Lawson Health Research Institute; Agriculture and Agri-Food Canada; Vineland Research and Innovation Centre; Western University","funders":"","keywords":"Recombinant DNA; Fusion protein; Pentapeptide repeat; FLAG-tag; Biochemistry; Myc-tag; Biology; Green fluorescent protein; Molecular biology; Chemistry; Peptide; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0006837444,0.0005649581,0.000662875,0.0002878381,0.0003060898,0.0005932542,0.0004230499,0.0004597647,0.000530508],"category_scores_gemma":[0.0003716866,0.0003541417,0.0004448777,0.0005627316,0.0002670351,0.0003699212,0.000416593,0.001092319,0.0005279842],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0006550648,"about_ca_system_score_gemma":0.0003056396,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0006958317,"about_ca_topic_score_gemma":0.001187274,"domain_scores_codex":[0.9995283,0.0001034705,0.00007720587,0.00008952025,0.0001326837,0.00006881354],"domain_scores_gemma":[0.999775,0.00006013687,0.00006613296,0.00002247391,0.00004173783,0.00003450898],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001929359,0.00001788566,0.00003373044,0.00002704415,0.000002807975,0.00002225907,0.00001774272,0.0001699431,0.9991382,0.00004102147,0.00001687622,0.0004930256],"study_design_scores_gemma":[0.00001017038,0.00008834568,0.0007505949,0.000008873692,0.00001468796,0.00009355622,0.00002851772,0.001755868,0.9944917,0.00004855994,0.002698951,0.00001005982],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9547094,0.001425774,0.0398991,0.0002406476,0.00005416412,0.0003521872,0.0009667671,0.0002683965,0.00208363],"genre_scores_gemma":[0.915315,0.002478052,0.0733142,0.0001823052,0.00002222955,0.0005808866,0.003116137,0.0003093554,0.004681904],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0006958317,"threshold_uncertainty_score":0.004752874,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.007288965561650111,"score_gpt":0.2129499761004593,"score_spread":0.2056610105388091,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}