{"id":"W2087743684","doi":"10.1385/mb:35:1:15","title":"Generation of linear expression constructs by one-step PCR with vaccinia DNA topoisomerase I","year":2007,"lang":"en","type":"article","venue":"Molecular Biotechnology","topic":"Bacteriophages and microbial interactions","field":"Environmental Science","cited_by":2,"is_retracted":false,"has_abstract":false,"ca_institutions":"Royal Ontario Museum","funders":"","keywords":"Biology; Molecular biology; Transfection; Chinese hamster ovary cell; Gene; Chloramphenicol acetyltransferase; Vaccinia; DNA; Topoisomerase; Plasmid; Expression vector; Terminator (solar); Gene expression; Cell culture; Reporter gene; Genetics; Recombinant DNA","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001518791,0.001946273,0.001568621,0.001039001,0.0007479595,0.001461369,0.001695007,0.00103008,0.00441655],"category_scores_gemma":[0.001183445,0.001695258,0.001469367,0.001041772,0.0009079648,0.001036478,0.0008987294,0.003806501,0.003769331],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.001148018,"about_ca_system_score_gemma":0.001032517,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001227786,"about_ca_topic_score_gemma":0.00117737,"domain_scores_codex":[0.9989963,0.0001211191,0.00013287,0.000243052,0.0002412482,0.0002653345],"domain_scores_gemma":[0.9987018,0.0005292991,0.0001561192,0.0001905566,0.0002244158,0.0001977793],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001620184,0.0001142961,0.0001230328,0.0001775571,0.00002397735,0.00007080722,0.00009518135,0.0002056898,0.9956703,0.0007962646,0.0003509943,0.002209801],"study_design_scores_gemma":[0.00005118033,0.0001159009,0.0003904381,0.00001610029,0.0000468667,0.00007278744,0.00003477686,0.001272833,0.9914512,0.0001504895,0.006380999,0.00001636377],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"methods","genre_scores_codex":[0.5384813,0.00218845,0.426453,0.001203109,0.001225008,0.003214743,0.01437278,0.004476807,0.008384741],"genre_scores_gemma":[0.6093828,0.002197979,0.3102379,0.0004864141,0.0001898592,0.001912343,0.03917018,0.002226269,0.03419618],"genre_candidate":"methods","genre_consensus":null,"teacher_disagreement_score":0.00441655,"threshold_uncertainty_score":0.01477486,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.009549702698924,"score_gpt":0.2277904065082473,"score_spread":0.2182407038093233,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}