{"id":"W2087929887","doi":"10.3791/1333","title":"&lt;em&gt;In utero&lt;/em&gt; and &lt;em&gt;ex vivo&lt;/em&gt; Electroporation for Gene Expression in Mouse Retinal Ganglion Cells","year":2009,"lang":"en","type":"article","venue":"Journal of Visualized Experiments","topic":"Retinal Development and Disorders","field":"Biochemistry, Genetics and Molecular Biology","cited_by":30,"is_retracted":false,"has_abstract":true,"ca_institutions":"Columbia College","funders":"National Institute of Neurological Disorders and Stroke; National Eye Institute; National Institutes of Health","keywords":"Electroporation; Biology; Retina; Retinal ganglion cell; Cell biology; Ex vivo; Axon guidance; Neuroscience; Axon; In vivo; Genetics; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0008744539,0.001173939,0.0006412758,0.001086486,0.0004891512,0.0008456994,0.0009288472,0.001245669,0.01025676],"category_scores_gemma":[0.0004460289,0.0008186568,0.0009514585,0.0005386195,0.001088018,0.0009619417,0.0006769606,0.002160095,0.0101527],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005168698,"about_ca_system_score_gemma":0.0004199386,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001012149,"about_ca_topic_score_gemma":0.00188656,"domain_scores_codex":[0.9993508,0.00007748897,0.0001029386,0.0001735056,0.0001806587,0.0001146184],"domain_scores_gemma":[0.9994251,0.0001094277,0.00016902,0.0001411185,0.0000631793,0.00009225265],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00005660635,0.00003289019,0.0001009006,0.0001131655,0.00000946016,0.0001562312,0.00004124646,0.0001504194,0.9923487,0.001393147,0.0007205859,0.004876651],"study_design_scores_gemma":[0.00004436439,0.0001397851,0.001972698,0.00005914607,0.00003694608,0.0006909955,0.00003901711,0.001134514,0.9530771,0.0003242992,0.04245927,0.00002196132],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.2909483,0.007924289,0.5794451,0.002570744,0.001806534,0.003537667,0.02474732,0.007939956,0.08108015],"genre_scores_gemma":[0.3839776,0.009077629,0.4414752,0.001527077,0.0003646216,0.003638804,0.01630498,0.00599237,0.1376417],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.01025676,"threshold_uncertainty_score":0.03431231,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01212733006554819,"score_gpt":0.3173728761307812,"score_spread":0.3052455460652331,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}