{"id":"W2087966510","doi":"10.1006/prep.2001.1401","title":"Expression and Purification of Escherichia coli β-Glucuronidase","year":2001,"lang":"en","type":"article","venue":"Protein Expression and Purification","topic":"Transgenic Plants and Applications","field":"Biochemistry, Genetics and Molecular Biology","cited_by":11,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Saskatchewan","funders":"","keywords":"Escherichia coli; Ammonium sulfate precipitation; Glucuronidase; Enzyme; Chemistry; Ion chromatography; Chromatography; Recombinant DNA; Size-exclusion chromatography; Biochemistry; Isocitrate dehydrogenase; Ammonium; Molecular biology; Biology; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004454071,0.0006790305,0.0006834707,0.0005317113,0.0004574387,0.0006269873,0.0005072158,0.0004253243,0.0009950271],"category_scores_gemma":[0.0004168985,0.0004459598,0.0004107675,0.001210745,0.0002592309,0.0002750973,0.0004078436,0.001358496,0.001791259],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0006212303,"about_ca_system_score_gemma":0.0007966848,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.002404131,"about_ca_topic_score_gemma":0.002622779,"domain_scores_codex":[0.999627,0.00004516215,0.00004076878,0.00006329665,0.0001369555,0.00008687244],"domain_scores_gemma":[0.9998259,0.00003078251,0.00002239034,0.00003322868,0.00005300684,0.0000347341],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00009281724,0.00006087011,0.0002472668,0.0001104431,0.00001036922,0.00007726941,0.00004866883,0.0001943913,0.9935661,0.0004448492,0.0004360726,0.004710861],"study_design_scores_gemma":[0.00002033601,0.00006060005,0.002466874,0.00001736151,0.00001860697,0.0002011208,0.00003227124,0.0009395145,0.9771606,0.0002327965,0.01883825,0.00001151483],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.718668,0.007673596,0.2405738,0.001769631,0.0004490266,0.00120208,0.01589805,0.001953627,0.01181223],"genre_scores_gemma":[0.6807306,0.01129375,0.2042475,0.0008084641,0.00009590897,0.0008474296,0.05501184,0.000602695,0.04636185],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.002404131,"threshold_uncertainty_score":0.004780293,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01360231744617094,"score_gpt":0.2484076578215549,"score_spread":0.2348053403753839,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}