{"id":"W2087966510","doi":"10.1006/prep.2001.1401","title":"Expression and Purification of Escherichia coli β-Glucuronidase","year":2001,"lang":"en","type":"article","venue":"Protein Expression and Purification","topic":"Transgenic Plants and Applications","field":"Biochemistry, Genetics and Molecular Biology","cited_by":11,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Saskatchewan","funders":"","keywords":"Escherichia coli; Ammonium sulfate precipitation; Glucuronidase; Enzyme; Chemistry; Ion chromatography; Chromatography; Recombinant DNA; Size-exclusion chromatography; Biochemistry; Isocitrate dehydrogenase; Ammonium; Molecular biology; Biology; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001474499,0.000122964,0.0001082921,0.00003808381,0.0001279424,0.00002306739,0.000101792,0.0001388459,0.00002367739],"category_scores_gemma":[0.00003064895,0.0001069171,0.00002655261,0.0000781461,0.00008139914,0.0000136961,0.00003597512,0.00006988247,0.000003025329],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000003635316,"about_ca_system_score_gemma":0.0000232639,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00001027543,"about_ca_topic_score_gemma":0.000002155648,"domain_scores_codex":[0.9991297,0.00006075414,0.0002335091,0.0003395761,0.0001097868,0.0001266821],"domain_scores_gemma":[0.9993775,0.000009090875,0.0001496956,0.000311766,0.00006048481,0.00009141986],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0000917977,0.00008375374,0.0006596738,0.00003156726,0.000004223401,1.778762e-7,0.00007286426,0.000002595006,0.9916068,0.0003565551,0.0002358252,0.0068542],"study_design_scores_gemma":[0.0004390223,0.00006386088,0.01040093,0.00005670073,0.000008634218,0.000004657284,0.00006605095,0.00008281997,0.949158,0.0002012296,0.03938594,0.0001322182],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.98622,0.001530834,0.01083292,0.0003175421,0.00001936892,0.0005492064,0.00002708014,0.00001891587,0.0004841091],"genre_scores_gemma":[0.9932981,0.001274067,0.004449004,0.00003860107,0.00005841592,0.0002073997,0.0001634814,0.00001360943,0.0004973009],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.04244883,"threshold_uncertainty_score":0.4359951,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01360231744617094,"score_gpt":0.2484076578215549,"score_spread":0.2348053403753839,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}