{"id":"W2088088087","doi":"10.1038/nprot.2007.21","title":"Synthesis of 3,3′-bis(sulfonato)-4,4′-bis(chloroacetamido)azobenzene and cysteine cross-linking for photo-control of protein conformation and activity","year":2007,"lang":"en","type":"article","venue":"Nature Protocols","topic":"Click Chemistry and Applications","field":"Chemistry","cited_by":63,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Toronto; Trent University","funders":"Natural Sciences and Engineering Research Council of Canada; Canadian Institutes of Health Research","keywords":"Azobenzene; Linker; Cysteine; Intramolecular force; Chemistry; In vitro; Combinatorial chemistry; Thiol; Disulfide bond; Stereochemistry; Biochemistry; Molecule; Organic chemistry; Enzyme; Computer science","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0006284544,0.0002143324,0.0004080223,0.00004778818,0.0001255685,0.00003173133,0.0001768913,0.0005647486,0.00002762803],"category_scores_gemma":[0.0004135496,0.00020334,0.0000906267,0.0001202101,0.0001334414,0.0001920871,0.0000533261,0.0003982459,2.061357e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00003657008,"about_ca_system_score_gemma":0.00006750741,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00001015397,"about_ca_topic_score_gemma":0.00000972841,"domain_scores_codex":[0.9986938,0.00001018947,0.0005056441,0.0003027752,0.0002426604,0.0002449265],"domain_scores_gemma":[0.9982824,0.0004381422,0.0005834188,0.0003441188,0.0002651636,0.00008677253],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.001163168,0.0001456218,0.001478922,0.002977261,0.00005287699,4.573835e-7,0.00005779356,0.000004063292,0.9825228,0.001362961,0.000008007647,0.01022606],"study_design_scores_gemma":[0.002340285,0.00005761768,0.0006270473,0.0005029981,0.00003849365,0.000009077065,0.00003434642,0.0004017477,0.9874552,0.0003333448,0.008014095,0.0001857121],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9638416,0.0001480408,0.002332492,0.00009284842,0.000006803961,0.02988866,0.0003715791,0.00005218462,0.003265858],"genre_scores_gemma":[0.9521223,0.000002984749,0.0008631919,0.00002076815,0.00008109505,0.04671616,0.00001555375,0.00002101231,0.000156925],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0168275,"threshold_uncertainty_score":0.8291966,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01071635001534703,"score_gpt":0.3185861968555672,"score_spread":0.3078698468402201,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}