{"id":"W2088088087","doi":"10.1038/nprot.2007.21","title":"Synthesis of 3,3′-bis(sulfonato)-4,4′-bis(chloroacetamido)azobenzene and cysteine cross-linking for photo-control of protein conformation and activity","year":2007,"lang":"en","type":"article","venue":"Nature Protocols","topic":"Click Chemistry and Applications","field":"Chemistry","cited_by":63,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Toronto; Trent University","funders":"Natural Sciences and Engineering Research Council of Canada; Canadian Institutes of Health Research","keywords":"Azobenzene; Linker; Cysteine; Intramolecular force; Chemistry; In vitro; Combinatorial chemistry; Thiol; Disulfide bond; Stereochemistry; Biochemistry; Molecule; Organic chemistry; Enzyme; Computer science","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0005286557,0.0006709241,0.0006024422,0.000314375,0.0004233521,0.0002663864,0.0008148301,0.000588378,0.002564557],"category_scores_gemma":[0.0002150146,0.0004714378,0.0004623917,0.0003544865,0.0002352856,0.0003875587,0.0003209237,0.0006582688,0.001176298],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0006070429,"about_ca_system_score_gemma":0.0008039124,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001038009,"about_ca_topic_score_gemma":0.002251698,"domain_scores_codex":[0.999741,0.00003474076,0.0000205333,0.00005822451,0.00005588182,0.00008967226],"domain_scores_gemma":[0.9998838,0.00001857582,0.0000278067,0.00001584646,0.00001717288,0.00003689222],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00008294803,0.00004957439,0.00006215277,0.0001129972,0.00001278267,0.0001875242,0.00005429846,0.0002118261,0.9949135,0.000766258,0.0002255632,0.003320614],"study_design_scores_gemma":[0.00002510338,0.0001235988,0.0003352282,0.000003256167,0.00001005885,0.0001493952,0.000007891916,0.0003465081,0.994126,0.00007844168,0.004783886,0.00001060688],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"methods","genre_scores_codex":[0.8765106,0.004315204,0.0996289,0.0006361555,0.0002558574,0.0007647197,0.001779692,0.001174996,0.01493378],"genre_scores_gemma":[0.9507787,0.001948251,0.0340899,0.0002168857,0.00003034827,0.0003561004,0.001452867,0.0001043313,0.01102259],"genre_candidate":"methods","genre_consensus":null,"teacher_disagreement_score":0.002564557,"threshold_uncertainty_score":0.008579254,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01071635001534703,"score_gpt":0.3185861968555672,"score_spread":0.3078698468402201,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}