{"id":"W2088235843","doi":"10.1016/j.pep.2013.12.005","title":"Expression and characterization of plant aspartic protease nepenthesin-1 from Nepenthes gracilis","year":2013,"lang":"en","type":"article","venue":"Protein Expression and Purification","topic":"Biochemical and Structural Characterization","field":"Biochemistry, Genetics and Molecular Biology","cited_by":41,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Calgary","funders":"Institute of Microbiology, Chinese Academy of Sciences; Natural Sciences and Engineering Research Council of Canada; Grantová Agentura České Republiky; Ministerstvo Školství, Mládeže a Tělovýchovy; Univerzita Karlova v Praze","keywords":"Proteases; Biology; Biochemistry; Protease; Enzyme; Heterologous expression; Carnivorous plant; Recombinant DNA; Glycosylation; Escherichia coli; Gene; Predation; Ecology","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001464557,0.000316186,0.0002346225,0.0002610957,0.0002084199,0.0003333525,0.0002548982,0.000262549,0.0004252697],"category_scores_gemma":[0.0001725411,0.0001271635,0.0002765539,0.0003471134,0.0001547266,0.0002744973,0.0001797488,0.0007079992,0.0003502335],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004409378,"about_ca_system_score_gemma":0.0002324183,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001172923,"about_ca_topic_score_gemma":0.001488166,"domain_scores_codex":[0.9999269,0.000008807049,0.00001090344,0.00001637656,0.00002514692,0.00001186843],"domain_scores_gemma":[0.9998835,0.00002690331,0.0000313577,0.00001703998,0.0000197947,0.00002137987],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003229087,0.00001590934,0.0001435298,0.00003631114,0.000002466662,0.00006475321,0.00002291019,0.00008789529,0.9987161,0.0000600631,0.0000226638,0.0007951879],"study_design_scores_gemma":[0.00001941635,0.0001223406,0.0189165,0.0000165198,0.00002833314,0.0009567346,0.00008587118,0.00161495,0.9682679,0.0001085107,0.009850148,0.00001294415],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9879087,0.001489366,0.006225185,0.0003236053,0.00003895476,0.00005949524,0.002379867,0.00008278708,0.001492011],"genre_scores_gemma":[0.9608493,0.001508881,0.01332429,0.0001681062,0.00001707223,0.00005811915,0.01566505,0.00006291254,0.008346262],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001172923,"threshold_uncertainty_score":0.003199279,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.008726141673046943,"score_gpt":0.1988515999897233,"score_spread":0.1901254583166764,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}