{"id":"W2088235843","doi":"10.1016/j.pep.2013.12.005","title":"Expression and characterization of plant aspartic protease nepenthesin-1 from Nepenthes gracilis","year":2013,"lang":"en","type":"article","venue":"Protein Expression and Purification","topic":"Biochemical and Structural Characterization","field":"Biochemistry, Genetics and Molecular Biology","cited_by":41,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Calgary","funders":"Institute of Microbiology, Chinese Academy of Sciences; Natural Sciences and Engineering Research Council of Canada; Grantová Agentura České Republiky; Ministerstvo Školství, Mládeže a Tělovýchovy; Univerzita Karlova v Praze","keywords":"Proteases; Biology; Biochemistry; Protease; Enzyme; Heterologous expression; Carnivorous plant; Recombinant DNA; Glycosylation; Escherichia coli; Gene; Predation; Ecology","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.00005900316,0.0001349,0.0001227873,0.00002953475,0.00007924815,0.00004485386,0.00007664849,0.0001482974,0.00007048357],"category_scores_gemma":[0.00006657394,0.0001041757,0.0000232929,0.0000398786,0.00006597769,0.00003497849,0.00006233295,0.00005716832,0.000003676289],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000003357424,"about_ca_system_score_gemma":0.0000122478,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00003060298,"about_ca_topic_score_gemma":5.946155e-7,"domain_scores_codex":[0.9991311,0.00007991862,0.0002425715,0.00031788,0.0001160434,0.0001125276],"domain_scores_gemma":[0.9994316,0.000009691134,0.000191696,0.0002044353,0.00006950781,0.00009303951],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00009647496,0.00003262954,0.001010047,0.00005189939,0.000004245929,9.41155e-8,0.00009260258,1.138242e-7,0.9920066,0.00001662932,0.00003428751,0.006654402],"study_design_scores_gemma":[0.0002565275,0.00005428605,0.03380148,0.0001185213,0.000006400989,0.000001441541,0.00002776565,0.0001236744,0.9643335,0.0002721946,0.0008759999,0.0001281619],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.997526,0.0002164975,0.001064367,0.0002406176,0.00002710876,0.0008007528,0.00009277229,0.00001437578,0.00001745623],"genre_scores_gemma":[0.9970359,0.0001767489,0.000972498,0.00004578634,0.00008162156,0.000240005,0.001319643,0.00001139264,0.000116446],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.03279143,"threshold_uncertainty_score":0.4248162,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.008726141673046943,"score_gpt":0.1988515999897233,"score_spread":0.1901254583166764,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}