{"id":"W2089181671","doi":"10.1002/cbic.201200340","title":"Heterologous Expression and Structural Characterisation of a Pyrazinone Natural Product Assembly Line","year":2012,"lang":"en","type":"article","venue":"ChemBioChem","topic":"Microbial Natural Products and Biosynthesis","field":"Medicine","cited_by":47,"is_retracted":false,"has_abstract":true,"ca_institutions":"McMaster University","funders":"Natural Sciences and Engineering Research Council of Canada; Canadian Institutes of Health Research","keywords":"Nonribosomal peptide; Stereochemistry; Enzyme; Chemistry; Heterologous expression; Peptide; Dipeptide; Natural product; Reductase; Combinatorial chemistry; Biosynthesis; Biochemistry; Gene; Recombinant DNA","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001711262,0.0001689343,0.0001360244,0.00008136556,0.00008748289,0.0002237152,0.0001775161,0.0001626865,0.0004779755],"category_scores_gemma":[0.0001782375,0.0001026514,0.0001497279,0.0001141509,0.000171179,0.0001332385,0.0001193829,0.0004609529,0.0003476914],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000281915,"about_ca_system_score_gemma":0.0001650997,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0005430857,"about_ca_topic_score_gemma":0.0005419943,"domain_scores_codex":[0.9999056,0.00001959463,0.00001031929,0.00001657142,0.00003525235,0.00001259536],"domain_scores_gemma":[0.9998623,0.00003589018,0.00004135736,0.00001906524,0.00001820384,0.00002307735],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001228319,0.000008483403,0.0000520602,0.000006350213,6.692591e-7,0.00002461846,0.000009387603,0.00007764911,0.9995422,0.00003696839,0.000003266918,0.0002259872],"study_design_scores_gemma":[0.000003693217,0.0000999497,0.0008695291,0.000001087886,0.000003378463,0.0001102216,0.00001606296,0.0006322825,0.9973862,0.00001301553,0.0008628813,0.000001768132],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9944055,0.0001723701,0.004566595,0.00005166287,0.00000521287,0.00001874987,0.0002157884,0.00002437532,0.000539685],"genre_scores_gemma":[0.9891003,0.0001972873,0.00787973,0.0000175324,0.000003581728,0.00001108198,0.001035268,0.00001974702,0.001735552],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0005430857,"threshold_uncertainty_score":0.002045453,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01416908321224418,"score_gpt":0.2566897737461158,"score_spread":0.2425206905338716,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}