{"id":"W2089181671","doi":"10.1002/cbic.201200340","title":"Heterologous Expression and Structural Characterisation of a Pyrazinone Natural Product Assembly Line","year":2012,"lang":"en","type":"article","venue":"ChemBioChem","topic":"Microbial Natural Products and Biosynthesis","field":"Medicine","cited_by":47,"is_retracted":false,"has_abstract":true,"ca_institutions":"McMaster University","funders":"Natural Sciences and Engineering Research Council of Canada; Canadian Institutes of Health Research","keywords":"Nonribosomal peptide; Stereochemistry; Enzyme; Chemistry; Heterologous expression; Peptide; Dipeptide; Natural product; Reductase; Combinatorial chemistry; Biosynthesis; Biochemistry; Gene; Recombinant DNA","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001282161,0.0001535124,0.0002814214,0.00005402835,0.00004080879,0.000008524886,0.00005285038,0.00009272176,0.00003686509],"category_scores_gemma":[0.0001744607,0.0001054349,0.0000543317,0.00009376284,0.00008278107,0.0001517673,0.00006494814,0.0001771162,0.000003343737],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00002249133,"about_ca_system_score_gemma":0.00001374955,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000007948297,"about_ca_topic_score_gemma":4.54909e-7,"domain_scores_codex":[0.9991587,0.00001650783,0.0002221857,0.0002339806,0.000131945,0.0002366984],"domain_scores_gemma":[0.9994203,0.00001748444,0.0001341027,0.0002295914,0.00009988763,0.00009866923],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001934241,0.00007321903,0.003221843,0.0001891009,0.0000224406,9.69809e-7,0.0002182243,1.583731e-8,0.9876949,0.000003126548,0.000293845,0.008088894],"study_design_scores_gemma":[0.0004060413,0.00007428053,0.09196373,0.0000732006,0.00004613321,0.0001034004,0.00002712406,0.00001481546,0.90689,0.000003628501,0.0002916662,0.0001060224],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9955475,0.002985086,0.000001116287,0.0008525396,0.0002641753,0.000255603,0.00001084221,0.00003637929,0.00004672689],"genre_scores_gemma":[0.9978853,0.00005381841,0.0007466984,0.0001787374,0.0007115643,9.281833e-7,0.0001109246,0.00001503187,0.0002969271],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.08874189,"threshold_uncertainty_score":0.4299511,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01416908321224418,"score_gpt":0.2566897737461158,"score_spread":0.2425206905338716,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}