{"id":"W2089478151","doi":"10.1042/bj20102131","title":"Recombinant Deg/HtrA proteases from <i>Synechocystis</i> sp. PCC 6803 differ in substrate specificity, biochemical characteristics and mechanism","year":2011,"lang":"en","type":"article","venue":"Biochemical Journal","topic":"Photosynthetic Processes and Mechanisms","field":"Biochemistry, Genetics and Molecular Biology","cited_by":31,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of British Columbia","funders":"","keywords":"Proteases; Synechocystis; Periplasmic space; Escherichia coli; Serine; Biochemistry; Recombinant DNA; Biology; PDZ domain; Enzyme; Cleavage (geology); Mutant; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["metaepi_narrow"],"consensus_categories":[],"category_scores_codex":[0.0002452419,0.000368027,0.000380292,0.00007057651,0.00009475181,0.00007855296,0.0004025591,0.0004273109,0.0001146525],"category_scores_gemma":[0.0001660683,0.0003242302,0.0001285633,0.0001006029,0.000146265,0.00001312246,0.0002260686,0.0004646196,0.00001046674],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00003981694,"about_ca_system_score_gemma":0.0001180735,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00003834247,"about_ca_topic_score_gemma":0.000004612908,"domain_scores_codex":[0.9979878,0.00006312086,0.0005672984,0.0005891732,0.0002431841,0.000549387],"domain_scores_gemma":[0.998917,0.00002173912,0.0002286967,0.0003201891,0.0001029051,0.0004094291],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0003423723,0.0003308537,0.0009818521,0.00002843033,0.00003212518,0.0000923503,0.00005888499,1.551809e-8,0.997005,0.00017529,0.0002578969,0.0006948741],"study_design_scores_gemma":[0.0009562353,0.0002551485,0.002549872,0.0001414976,0.0000426548,0.0004522245,0.00007387314,0.00001688323,0.9855589,0.008896949,0.0006231646,0.0004325488],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9964591,0.0006200828,0.001923078,0.00008220156,0.0003667196,0.0002465771,0.000115891,0.00001872779,0.0001675921],"genre_scores_gemma":[0.995261,0.001500484,0.002404912,0.0002183674,0.0003834565,0.00002632505,0.0001095864,0.00004715937,0.00004876774],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.01144611,"threshold_uncertainty_score":0.999921,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01796381629805387,"score_gpt":0.2147973468880952,"score_spread":0.1968335305900414,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}