{"id":"W2089749430","doi":"10.1021/pr801008c","title":"A Carboxy-Terminal Affinity Tag for the Purification and Mass Spectrometric Characterization of Integral Membrane Proteins","year":2009,"lang":"en","type":"article","venue":"Journal of Proteome Research","topic":"Receptor Mechanisms and Signaling","field":"Biochemistry, Genetics and Molecular Biology","cited_by":36,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of British Columbia","funders":"Canadian Institutes of Health Research","keywords":"Integral membrane protein; Membrane protein; Biochemistry; G protein-coupled receptor; Chemistry; ATP-binding cassette transporter; Peptide; Trypsin; Biology; Membrane; Enzyme; Receptor; Transporter; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004091538,0.0008152191,0.0005592302,0.0007403966,0.000416151,0.0004325682,0.0005920474,0.000613265,0.00135531],"category_scores_gemma":[0.000490328,0.0003217346,0.000475149,0.0007055256,0.0002701526,0.0003760233,0.0005511556,0.0007501928,0.001526828],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004943855,"about_ca_system_score_gemma":0.0006239292,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0007067661,"about_ca_topic_score_gemma":0.001077948,"domain_scores_codex":[0.9995664,0.00009572819,0.00004743334,0.00007428783,0.0001562215,0.00005998526],"domain_scores_gemma":[0.9997882,0.00004935344,0.00004674584,0.00003794196,0.00004972283,0.00002807361],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002396973,0.00001832975,0.0000946306,0.00008108384,0.000009541259,0.00003186263,0.00000603272,0.00003395009,0.996816,0.00008367049,0.0001013281,0.002699597],"study_design_scores_gemma":[0.00001200832,0.00008074513,0.001785975,0.00001324076,0.00004034498,0.0006383912,0.00001023572,0.001145119,0.984329,0.00009985184,0.01183385,0.00001124],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"methods","genre_scores_codex":[0.6338848,0.01333016,0.3389574,0.001102432,0.0003134963,0.0006629223,0.005029048,0.001107839,0.005611804],"genre_scores_gemma":[0.5606167,0.01401449,0.3923253,0.0007545946,0.0001269572,0.0006946051,0.01924739,0.0003156909,0.01190432],"genre_candidate":"methods","genre_consensus":null,"teacher_disagreement_score":0.00135531,"threshold_uncertainty_score":0.004534006,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.04270834688031386,"score_gpt":0.3350285776218639,"score_spread":0.2923202307415501,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}