{"id":"W2089877124","doi":"10.1002/prot.22446","title":"Engineering monomeric streptavidin and its ligands with infinite affinity in binding but reversibility in interaction","year":2009,"lang":"en","type":"article","venue":"Proteins Structure Function and Bioinformatics","topic":"Biotin and Related Studies","field":"Biochemistry, Genetics and Molecular Biology","cited_by":14,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Calgary","funders":"Wayne State University","keywords":"Biotinylation; Streptavidin; Biotin; Chemistry; Cysteine; Peptide; Monomer; Protein engineering; Residue (chemistry); Combinatorial chemistry; Biochemistry; Enzyme; Organic chemistry","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004773117,0.0004832856,0.0003285611,0.0002201107,0.0001549821,0.0005649237,0.0006089892,0.0006280087,0.0007244411],"category_scores_gemma":[0.0003862002,0.0003049178,0.0002742535,0.0002576908,0.0002985385,0.0003631517,0.0004901119,0.0005586421,0.0009979963],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005778557,"about_ca_system_score_gemma":0.000388392,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0004156509,"about_ca_topic_score_gemma":0.0007544506,"domain_scores_codex":[0.9995497,0.0001312524,0.00004000893,0.00007201648,0.0001288501,0.00007812984],"domain_scores_gemma":[0.9998072,0.00003442748,0.00004032803,0.00002220843,0.00004755847,0.00004838445],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00005456847,0.00003905628,0.000159128,0.00006426029,0.000006736382,0.00004898544,0.00002606865,0.0005428786,0.9886684,0.002676399,0.0001297544,0.007583781],"study_design_scores_gemma":[0.0000167362,0.0002291595,0.0002326777,0.000007364788,0.00001311051,0.0002618981,0.00002214061,0.004680751,0.9841226,0.000550285,0.009852014,0.00001124308],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.7563772,0.006244728,0.2214281,0.0007589193,0.0001815385,0.0003154575,0.0001972003,0.0009119138,0.01358496],"genre_scores_gemma":[0.8703079,0.001660729,0.1183575,0.000257138,0.00002625478,0.0002063346,0.0002839732,0.00008658844,0.008813623],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0007244411,"threshold_uncertainty_score":0.00419265,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.005893303231245437,"score_gpt":0.1990218540328472,"score_spread":0.1931285508016017,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}