{"id":"W2090118739","doi":"10.1139/o2012-006","title":"Random mutagenesis suggests that sequence errors are not a major cause of variation in the activity of individual molecules of β-galactosidase","year":2012,"lang":"en","type":"article","venue":"Biochemistry and Cell Biology","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":12,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Winnipeg","funders":"Alexander von Humboldt-Stiftung","keywords":"Plasmid; Beta-galactosidase; Escherichia coli; Biology; Mutagenesis; Genetics; Molecular biology; lac operon; Population; Gene; Directed evolution; Enzyme; Mutation; Biochemistry; Mutant","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":true,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001453437,0.0005486803,0.0006842994,0.0003561866,0.000174694,0.0006792056,0.0003689215,0.0004908899,0.001119578],"category_scores_gemma":[0.003582753,0.0003245429,0.0002915768,0.0003068507,0.0005616267,0.0004045039,0.0003715343,0.0009085078,0.000583574],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003089513,"about_ca_system_score_gemma":0.0004336873,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0004022756,"about_ca_topic_score_gemma":0.0009704737,"domain_scores_codex":[0.998187,0.0004091455,0.0002173274,0.000508778,0.0005284503,0.0001492836],"domain_scores_gemma":[0.9968435,0.001348587,0.0006422165,0.0006517158,0.0003997973,0.0001143335],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001305899,0.00006315584,0.00410236,0.0001343451,0.00005686074,0.0001643918,0.00004764495,0.0006161491,0.9883409,0.000681256,0.00008085441,0.005581416],"study_design_scores_gemma":[0.00001931331,0.0003526743,0.01981461,0.00003146426,0.0001025612,0.002038159,0.00006582861,0.005962645,0.9667667,0.001170848,0.003643943,0.00003125626],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.912375,0.0006134272,0.08408569,0.000266365,0.00006925133,0.0001348602,0.0004475372,0.0003551247,0.00165267],"genre_scores_gemma":[0.9789069,0.0003606661,0.01848434,0.0001589154,0.000009768732,0.00006356565,0.0007276064,0.000165264,0.001123002],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001453437,"threshold_uncertainty_score":0.007686615,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01711816765037375,"score_gpt":0.2874540338992204,"score_spread":0.2703358662488466,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}