{"id":"W2090727866","doi":"10.1016/s0168-1656(01)00428-x","title":"Design of high essential amino acid proteins: two design strategies for improving protease resistance of the nutritious MB-1 protein","year":2002,"lang":"en","type":"article","venue":"Journal of Biotechnology","topic":"Transgenic Plants and Applications","field":"Biochemistry, Genetics and Molecular Biology","cited_by":6,"is_retracted":false,"has_abstract":false,"ca_institutions":"Université du Québec à Trois-Rivières","funders":"Natural Sciences and Engineering Research Council of Canada; Dairy Farmers of Canada","keywords":"Mutant; Protein design; Protease; Protein engineering; Amino acid; Biochemistry; Denaturation (fissile materials); Mutagenesis; Protein folding; Protein structure; Biology; Chemistry; Enzyme","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002456805,0.0001260987,0.0002404336,0.00007565607,0.00007220307,0.00001096954,0.0004551743,0.000257633,0.000005408429],"category_scores_gemma":[0.00008472426,0.00009192444,0.0001195278,0.0001352859,0.0002567593,0.000009443982,0.00003703919,0.0001752661,2.591952e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0000119435,"about_ca_system_score_gemma":0.0001427954,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000009676225,"about_ca_topic_score_gemma":0.00001398242,"domain_scores_codex":[0.9989636,0.00007377439,0.0004835261,0.0001690986,0.0001257871,0.00018421],"domain_scores_gemma":[0.9988188,0.000016629,0.0006611319,0.0003203893,0.0001544733,0.00002863898],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002936438,0.0001821534,0.000002185696,0.0001017172,0.00005723481,0.000001992425,0.00002107562,0.0001525863,0.996402,0.001259682,0.0002158635,0.001309921],"study_design_scores_gemma":[0.001102046,0.0006697629,0.0000168096,0.00007042946,0.00004610022,0.00004509327,0.00006511035,0.00008585577,0.9945211,0.00282435,0.0004589721,0.00009431653],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"empirical","genre_scores_codex":[0.3843811,0.001230238,0.6116838,0.001069533,0.00003877127,0.001526979,0.00005465481,0.000007902801,0.000007066812],"genre_scores_gemma":[0.9431591,0.00009432698,0.05645384,0.00001568447,0.00007272303,0.0001232331,0.000001943121,0.00001364723,0.000065509],"genre_candidate":"empirical","genre_consensus":null,"teacher_disagreement_score":0.558778,"threshold_uncertainty_score":0.374857,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01497294366781079,"score_gpt":0.2304011648384096,"score_spread":0.2154282211705988,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}