{"id":"W2090937355","doi":"10.1016/j.vaccine.2009.10.028","title":"Improving the expression of recombinant soluble HIV Envelope glycoproteins using pseudo-stable transient transfection","year":2009,"lang":"en","type":"article","venue":"Vaccine","topic":"Monoclonal and Polyclonal Antibodies Research","field":"Medicine","cited_by":53,"is_retracted":false,"has_abstract":false,"ca_institutions":"","funders":"National Institute of Allergy and Infectious Diseases; National Research Council Canada; Bill and Melinda Gates Foundation","keywords":"Immunogenicity; Glycoprotein; Transfection; Virology; Recombinant DNA; Antibody; Biology; Virus; Neutralizing antibody; Viral envelope; Cell culture; Gene; Molecular biology; Immunology; Biochemistry; Genetics","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0008101422,0.0006690245,0.0005538128,0.0004390866,0.0003379575,0.0009707804,0.0005886694,0.0006303642,0.001363956],"category_scores_gemma":[0.0005711137,0.0003659204,0.0006176885,0.0004970872,0.0004543315,0.0005284728,0.0005770148,0.001666744,0.001076666],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0006859266,"about_ca_system_score_gemma":0.0004246538,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0008511632,"about_ca_topic_score_gemma":0.001320493,"domain_scores_codex":[0.9994171,0.0001390718,0.00007759164,0.00007880114,0.0001970324,0.00009049167],"domain_scores_gemma":[0.9997417,0.00007673205,0.00004707341,0.0000536158,0.00005836437,0.00002242345],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003647546,0.00002591633,0.00006536992,0.00004677462,0.000007350519,0.00003372425,0.00002406084,0.0001089145,0.9970092,0.0004351485,0.0001253699,0.002081673],"study_design_scores_gemma":[0.000009165869,0.00006558019,0.0002834011,0.000004300216,0.00002085835,0.0001357547,0.000009936447,0.001126018,0.9940995,0.00005659806,0.004183792,0.00000516463],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8057998,0.00460033,0.1802099,0.0008674985,0.0003698521,0.000402915,0.001432279,0.001290647,0.005026764],"genre_scores_gemma":[0.9130989,0.003399175,0.06634416,0.0002299983,0.00006408957,0.0002839536,0.003105842,0.0003714045,0.0131024],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001363956,"threshold_uncertainty_score":0.004976809,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02584507530469239,"score_gpt":0.2846938913508374,"score_spread":0.2588488160461451,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}