{"id":"W2090937355","doi":"10.1016/j.vaccine.2009.10.028","title":"Improving the expression of recombinant soluble HIV Envelope glycoproteins using pseudo-stable transient transfection","year":2009,"lang":"en","type":"article","venue":"Vaccine","topic":"Monoclonal and Polyclonal Antibodies Research","field":"Medicine","cited_by":53,"is_retracted":false,"has_abstract":false,"ca_institutions":"","funders":"National Institute of Allergy and Infectious Diseases; National Research Council Canada; Bill and Melinda Gates Foundation","keywords":"Immunogenicity; Glycoprotein; Transfection; Virology; Recombinant DNA; Antibody; Biology; Virus; Neutralizing antibody; Viral envelope; Cell culture; Gene; Molecular biology; Immunology; Biochemistry; Genetics","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003519621,0.0001590252,0.0003121011,0.000110009,0.0002150207,0.00001496762,0.0001211047,0.00007745788,0.0002819801],"category_scores_gemma":[0.00003194215,0.00009380969,0.0001378552,0.0002968408,0.00001539595,0.0001061618,0.00002216823,0.0002671663,0.00001035571],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00005312972,"about_ca_system_score_gemma":0.0001014175,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0005816981,"about_ca_topic_score_gemma":0.0000181228,"domain_scores_codex":[0.9986331,0.00005803572,0.0003515768,0.0002440091,0.0003567988,0.0003565147],"domain_scores_gemma":[0.9993825,0.00004462281,0.00007253698,0.0002682587,0.0001180411,0.000114067],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.001264962,0.000286084,0.001366579,0.0001572617,0.00001992942,0.00001876954,0.0003469444,0.00009404664,0.9767787,0.0001002357,0.001124597,0.01844191],"study_design_scores_gemma":[0.004194184,0.001902321,0.04171805,0.0006464024,0.0001301956,0.0002688053,0.0002127768,0.01222681,0.9235729,0.0002428342,0.01459957,0.0002851519],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9888713,0.000595113,0.004946861,0.00391905,0.0002654771,0.0006603799,0.000003707189,0.00003634087,0.0007017915],"genre_scores_gemma":[0.9936718,0.0002312178,0.002063878,0.0001679808,0.0001477569,0.000009193024,0.00001317834,0.00001634692,0.003678632],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.05320578,"threshold_uncertainty_score":0.3825448,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02584507530469239,"score_gpt":0.2846938913508374,"score_spread":0.2588488160461451,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}