{"id":"W2091825484","doi":"10.3791/51887","title":"Immunodetection of Outer Membrane Proteins by Flow Cytometry of Isolated Mitochondria","year":2014,"lang":"en","type":"article","venue":"Journal of Visualized Experiments","topic":"Mitochondrial Function and Pathology","field":"Biochemistry, Genetics and Molecular Biology","cited_by":8,"is_retracted":false,"has_abstract":true,"ca_institutions":"Université de Montréal","funders":"Canadian Institutes of Health Research; ALS Society of Canada","keywords":"Mitochondrion; Flow cytometry; Biology; Organelle; Cell biology; Staining; Molecular biology; Biochemistry","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002793449,0.0001451537,0.0003788121,0.0001452557,0.00003087072,0.000008009143,0.0001757287,0.0002090606,0.0001522692],"category_scores_gemma":[0.0001537098,0.0001247811,0.0002101449,0.0001290091,0.00006013575,0.00001021664,0.00004461433,0.0001134625,0.000003951598],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001383311,"about_ca_system_score_gemma":0.00003752391,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00001051255,"about_ca_topic_score_gemma":5.70912e-7,"domain_scores_codex":[0.9985449,0.000226713,0.0006996292,0.000156108,0.0002270804,0.0001455553],"domain_scores_gemma":[0.9987057,0.00001463889,0.000707318,0.0002316008,0.0002682354,0.00007250169],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.001803395,0.0002494043,0.0001160059,0.00002519492,0.0001521151,0.000001178263,0.0001335161,0.00001545022,0.9952757,0.00000616457,0.00165063,0.0005712073],"study_design_scores_gemma":[0.002924786,0.002278085,0.00003600841,0.00004019693,0.00003023547,0.00005709732,0.00005952096,0.0001478481,0.9829166,0.00001033061,0.0113915,0.000107797],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8856739,0.0008411117,0.1116218,0.00002371265,0.001319996,0.0002002823,0.00001403931,0.000004853664,0.0003002898],"genre_scores_gemma":[0.9914588,0.000119847,0.007519235,0.00009460874,0.0002606949,0.000007289382,0.00002897549,0.00002484545,0.0004857315],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.1057849,"threshold_uncertainty_score":0.5088425,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01142428428678686,"score_gpt":0.3389832138407291,"score_spread":0.3275589295539422,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}