{"id":"W2091825484","doi":"10.3791/51887","title":"Immunodetection of Outer Membrane Proteins by Flow Cytometry of Isolated Mitochondria","year":2014,"lang":"en","type":"article","venue":"Journal of Visualized Experiments","topic":"Mitochondrial Function and Pathology","field":"Biochemistry, Genetics and Molecular Biology","cited_by":8,"is_retracted":false,"has_abstract":true,"ca_institutions":"Université de Montréal","funders":"Canadian Institutes of Health Research; ALS Society of Canada","keywords":"Mitochondrion; Flow cytometry; Biology; Organelle; Cell biology; Staining; Molecular biology; Biochemistry","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0007236024,0.001264881,0.0008697598,0.001839175,0.001022381,0.0008777062,0.001111315,0.0008806641,0.005885821],"category_scores_gemma":[0.0005653565,0.0003632996,0.0006400626,0.0008750791,0.0005849196,0.001050401,0.0005612589,0.003205232,0.002416932],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0007405592,"about_ca_system_score_gemma":0.0005808594,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0005176148,"about_ca_topic_score_gemma":0.00110092,"domain_scores_codex":[0.9994065,0.00006568044,0.00006213508,0.0001922988,0.0001641638,0.0001092331],"domain_scores_gemma":[0.9994817,0.0001751493,0.00006430426,0.00007649753,0.0001478559,0.00005445339],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003334599,0.00003231354,0.00004638189,0.00009870956,0.000005359541,0.00002447951,0.00002861724,0.00006399437,0.9960367,0.0004445376,0.0001616975,0.003023898],"study_design_scores_gemma":[0.00002941954,0.0001052441,0.001095103,0.00003679621,0.00002018848,0.0001449595,0.00002747771,0.003357029,0.9855973,0.0006745355,0.008897992,0.00001390842],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.2566149,0.005966782,0.7131333,0.001057502,0.001133597,0.001492401,0.006007365,0.004270493,0.01032372],"genre_scores_gemma":[0.2761604,0.01537629,0.6736736,0.0008851636,0.0005101071,0.004268289,0.01507921,0.001195179,0.01285195],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.005885821,"threshold_uncertainty_score":0.01968998,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01142428428678686,"score_gpt":0.3389832138407291,"score_spread":0.3275589295539422,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}