{"id":"W2091917976","doi":"10.1002/chem.200204551","title":"Mutations in Distant Residues Moderately Increase the Enantioselectivity of <i>Pseudomonas fluorescens</i> Esterase towards Methyl 3Bromo‐2‐methylpropanoate and Ethyl 3Phenylbutyrate","year":2003,"lang":"en","type":"article","venue":"Chemistry - A European Journal","topic":"Enzyme Catalysis and Immobilization","field":"Biochemistry, Genetics and Molecular Biology","cited_by":95,"is_retracted":false,"has_abstract":true,"ca_institutions":"McGill University","funders":"","keywords":"Saturated mutagenesis; Directed evolution; Pseudomonas fluorescens; Mutagenesis; Active site; Mutant; Site-directed mutagenesis; Esterase; Homology modeling; Point mutation; Mutation; Biochemistry; Genetics; Biology; Chemistry; Stereochemistry; Enzyme; Gene; Bacteria","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001355007,0.0002198469,0.0002265407,0.00003767418,0.0001985753,0.0001009206,0.000207004,0.00006319661,0.00002039213],"category_scores_gemma":[0.000708006,0.0001635843,0.0001061959,0.0002105439,0.0002073326,0.00002040376,0.00007854398,0.00031097,0.000001285295],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00002284986,"about_ca_system_score_gemma":0.0001684548,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00001286983,"about_ca_topic_score_gemma":0.00001079757,"domain_scores_codex":[0.9979988,0.0007253787,0.0004974069,0.0003380956,0.0001808543,0.000259471],"domain_scores_gemma":[0.9989923,0.00003337302,0.0002830852,0.0003402349,0.000212425,0.0001386003],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.000104222,0.0001073241,0.0007570238,0.00004363645,0.00004859656,0.00004417995,0.0002045053,0.0001869443,0.9971286,0.0000144666,0.0001587724,0.001201705],"study_design_scores_gemma":[0.0008718449,0.00008428386,0.002316226,0.00005147335,0.00005262137,0.0002824407,0.0002224608,0.0002043858,0.993766,0.0001261922,0.001816598,0.0002054587],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9947638,0.001561958,0.002001388,0.0000851821,0.00004289239,0.0001074157,0.00002432074,0.000006427654,0.001406632],"genre_scores_gemma":[0.998049,0.0007296487,0.0005964768,0.00006237575,0.00008085657,0.000005585111,0.00006835495,0.00003525813,0.0003725037],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.003362609,"threshold_uncertainty_score":0.6670774,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01208438283872098,"score_gpt":0.236491238948659,"score_spread":0.224406856109938,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}