{"id":"W2091917976","doi":"10.1002/chem.200204551","title":"Mutations in Distant Residues Moderately Increase the Enantioselectivity of <i>Pseudomonas fluorescens</i> Esterase towards Methyl 3Bromo‐2‐methylpropanoate and Ethyl 3Phenylbutyrate","year":2003,"lang":"en","type":"article","venue":"Chemistry - A European Journal","topic":"Enzyme Catalysis and Immobilization","field":"Biochemistry, Genetics and Molecular Biology","cited_by":95,"is_retracted":false,"has_abstract":true,"ca_institutions":"McGill University","funders":"","keywords":"Saturated mutagenesis; Directed evolution; Pseudomonas fluorescens; Mutagenesis; Active site; Mutant; Site-directed mutagenesis; Esterase; Homology modeling; Point mutation; Mutation; Biochemistry; Genetics; Biology; Chemistry; Stereochemistry; Enzyme; Gene; Bacteria","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002090911,0.0005704274,0.0002586601,0.0002349305,0.0001101007,0.0003291467,0.0002533933,0.0003051281,0.0008514804],"category_scores_gemma":[0.0003906804,0.0002091683,0.0003531831,0.0001655213,0.0002240006,0.0001585414,0.0003046531,0.0004746796,0.0003559534],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003000065,"about_ca_system_score_gemma":0.0002155372,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001006836,"about_ca_topic_score_gemma":0.001712202,"domain_scores_codex":[0.9996579,0.00005620405,0.00005157844,0.00004788827,0.000117081,0.00006940717],"domain_scores_gemma":[0.9996706,0.00008911745,0.0001025792,0.00003177465,0.00003000456,0.00007588255],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00005899784,0.00003034809,0.0003458269,0.00001138109,0.000006481033,0.0000582682,0.000006594272,0.0001025932,0.9984419,0.00002005492,0.00001247317,0.0009051186],"study_design_scores_gemma":[0.00001450044,0.0001796733,0.003569232,0.000003099902,0.00001435613,0.0003105655,0.0000174892,0.0006976243,0.9947698,0.00001898337,0.0003992384,0.00000556427],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9981321,0.0001118108,0.0009752979,0.00003916561,0.00000530629,0.00001016096,0.00007348871,0.00005304978,0.0005996499],"genre_scores_gemma":[0.9978608,0.00009737947,0.001361291,0.00003375862,0.000002950429,0.000007879951,0.0001754238,0.00002940924,0.0004309194],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001006836,"threshold_uncertainty_score":0.002848506,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01208438283872098,"score_gpt":0.236491238948659,"score_spread":0.224406856109938,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}