{"id":"W2093760833","doi":"10.1016/j.ymthe.2004.11.012","title":"eGFP reporter genes silence LCRβ-globin transgene expression via CpG dinucleotides","year":2004,"lang":"en","type":"article","venue":"Molecular Therapy","topic":"Virus-based gene therapy research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":31,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Toronto; Hospital for Sick Children","funders":"National Heart, Lung, and Blood Institute; Canadian Institutes of Health Research; National Institutes of Health; Stem Cell Network","keywords":"Biology; Molecular biology; Locus control region; CpG site; Gene silencing; Transgene; Globin; Green fluorescent protein; Gene; Reporter gene; Coding region; DNA methylation; Retrovirus; Promoter; Gene expression; Genetics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["metaepi_narrow"],"consensus_categories":[],"category_scores_codex":[0.000352208,0.0003761089,0.000263827,0.00009572089,0.0001704588,0.00006096651,0.0005397535,0.0002438545,0.0002062316],"category_scores_gemma":[0.00001799962,0.0003414094,0.0002774665,0.0002071251,0.0001717453,0.00001082853,0.00007956546,0.000159047,0.00004898963],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00004149818,"about_ca_system_score_gemma":0.0001504003,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00003039986,"about_ca_topic_score_gemma":0.00002566745,"domain_scores_codex":[0.9975639,0.0001738952,0.0003839179,0.0008057115,0.0004774984,0.0005950504],"domain_scores_gemma":[0.9984715,0.000007368811,0.0001154898,0.001063819,0.0001352032,0.0002065966],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00133275,0.0001866467,0.000153422,0.000009981844,0.00009770948,0.0001137389,0.00008236961,0.00107652,0.9677379,0.00001471657,0.0001185939,0.02907562],"study_design_scores_gemma":[0.001906313,0.002058178,0.0002726008,0.00002621165,0.000008078684,0.00005824548,0.00002667544,0.00001381294,0.9862817,0.0007136791,0.008216847,0.0004176949],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9637755,0.008119027,0.02675528,0.0003519706,0.0001285857,0.0005016727,0.00001356432,0.00005722287,0.0002972163],"genre_scores_gemma":[0.9945113,0.00194585,0.001516619,0.00119145,0.0001945043,0.0001616202,0.00009988392,0.0001088947,0.0002698604],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.03073586,"threshold_uncertainty_score":0.9999038,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01548457020222051,"score_gpt":0.2839236692086278,"score_spread":0.2684390990064073,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}