{"id":"W2094714066","doi":"10.1177/1087057105275344","title":"High-Throughput Screening of G Protein-Coupled Receptor Antagonists Using a Bioluminescence Resonance Energy Transfer 1-Based β-Arrestin2 Recruitment Assay","year":2005,"lang":"en","type":"article","venue":"SLAS DISCOVERY","topic":"Receptor Mechanisms and Signaling","field":"Biochemistry, Genetics and Molecular Biology","cited_by":178,"is_retracted":false,"has_abstract":false,"ca_institutions":"McGill University; Université de Montréal","funders":"","keywords":"G protein-coupled receptor; Agonist; Receptor; Bioluminescence; HEK 293 cells; Chemokine receptor; Cell biology; High-throughput screening; Biology; Chemistry; Biochemistry; Chemokine","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0007573467,0.001185387,0.001636781,0.0009979735,0.0005679728,0.0007343566,0.000953374,0.0006661469,0.00138724],"category_scores_gemma":[0.0003905661,0.0004983238,0.0007488522,0.0007756053,0.0002797038,0.0002799568,0.000506975,0.001151902,0.001055193],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0007374705,"about_ca_system_score_gemma":0.0005010372,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001069918,"about_ca_topic_score_gemma":0.004243011,"domain_scores_codex":[0.999113,0.0001785296,0.00005956322,0.0001168295,0.0004215124,0.0001105079],"domain_scores_gemma":[0.9998017,0.0000519402,0.00003688479,0.00002218898,0.00005009041,0.00003733848],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001145424,0.0002216438,0.0001760287,0.00004398518,0.00002485644,0.00004008469,0.0000112903,0.0004388724,0.9962538,0.00005845101,0.000261732,0.002354785],"study_design_scores_gemma":[0.000176063,0.001704886,0.00186132,0.000007178354,0.00008988193,0.0003231082,0.00002710757,0.006144939,0.9852573,0.00005420457,0.004323437,0.00003059137],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9377669,0.002712896,0.0478133,0.0005854419,0.0000846683,0.001631329,0.003917294,0.001116435,0.004371747],"genre_scores_gemma":[0.9186226,0.004091751,0.05705103,0.0003391018,0.00005335702,0.001490927,0.007904651,0.0002099101,0.01023677],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001636781,"threshold_uncertainty_score":0.005350769,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02838381151095562,"score_gpt":0.2617637164937525,"score_spread":0.2333799049827969,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}