{"id":"W2094729803","doi":"10.3791/2763","title":"DNA Extraction from Paraffin Embedded Material for Genetic and Epigenetic Analyses","year":2011,"lang":"en","type":"article","venue":"Journal of Visualized Experiments","topic":"Molecular Biology Techniques and Applications","field":"Biochemistry, Genetics and Molecular Biology","cited_by":131,"is_retracted":false,"has_abstract":true,"ca_institutions":"BC Cancer Agency; Occupational Cancer Research Centre; University of British Columbia","funders":"Canadian Institutes of Health Research","keywords":"DNA extraction; Lysis buffer; DNA microarray; DNA; Biology; Proteinase K; Nucleic acid; Lysis; Epigenetics; DNA methylation; Sodium dodecyl sulfate; Molecular biology; Polymerase chain reaction; Chromatography; Gene; Chemistry; Biochemistry; Gene expression","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.002911465,0.002152384,0.002144645,0.007789903,0.001303405,0.0008803147,0.002196602,0.001966773,0.03680713],"category_scores_gemma":[0.002546888,0.001076097,0.001552194,0.003737195,0.001023964,0.001196058,0.001280466,0.00297516,0.03317305],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003202522,"about_ca_system_score_gemma":0.00152311,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0006380067,"about_ca_topic_score_gemma":0.001993655,"domain_scores_codex":[0.9983247,0.0002340455,0.0003263073,0.0004931646,0.0004710939,0.0001507786],"domain_scores_gemma":[0.9972613,0.0004828565,0.0002232716,0.000724587,0.00109516,0.0002128133],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0008465003,0.0005285579,0.003647357,0.004325942,0.0001988506,0.005505234,0.0003070989,0.000586894,0.8744276,0.002205186,0.01823408,0.08918669],"study_design_scores_gemma":[0.0008426345,0.003342265,0.02769495,0.00342273,0.0008997573,0.01236733,0.0006970812,0.004742994,0.3579462,0.008597909,0.5791302,0.0003159596],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.06326857,0.0275245,0.7467489,0.001263836,0.004861266,0.04575821,0.05883648,0.009809025,0.04192909],"genre_scores_gemma":[0.05209065,0.02366003,0.8002827,0.00221492,0.001297154,0.02875703,0.06468248,0.001544793,0.02547027],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.03680713,"threshold_uncertainty_score":0.1231321,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.06171041381074732,"score_gpt":0.4589322119794625,"score_spread":0.3972217981687152,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}