{"id":"W2095218887","doi":"10.1016/j.jim.2004.09.001","title":"A simple and rapid protocol for the sequence determination of functional kappa light chain cDNAs from aberrant-chain-positive murine hybridomas","year":2004,"lang":"en","type":"article","venue":"Journal of Immunological Methods","topic":"Monoclonal and Polyclonal Antibodies Research","field":"Medicine","cited_by":21,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Manitoba; Canadian Science Centre for Human and Animal Health; Health Canada","funders":"Health Canada; Canadian Food Inspection Agency","keywords":"Kappa; Immunoglobulin light chain; Primer (cosmetics); Complementary DNA; Biology; Molecular biology; Polymerase chain reaction; GenBank; Inverse polymerase chain reaction; Genetics; Sequence analysis; Gene; Antibody; Chemistry; Nested polymerase chain reaction","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001653279,0.0001661382,0.0004935855,0.0001136777,0.0001559514,0.00001983658,0.0001639753,0.0001111926,0.0001666356],"category_scores_gemma":[0.001182028,0.00008485425,0.0002502197,0.0001472619,0.0003159215,0.00009134087,0.00008883088,0.0004645467,0.000001051203],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00007670374,"about_ca_system_score_gemma":0.0001509089,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00005325998,"about_ca_topic_score_gemma":0.000001913912,"domain_scores_codex":[0.9982463,0.0003450404,0.0006238041,0.0001763209,0.0003743016,0.0002342409],"domain_scores_gemma":[0.9967576,0.002081956,0.0004230515,0.0001428924,0.0004954808,0.0000989653],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.009069693,0.0003747213,0.0003833662,0.00009971749,0.0003096645,0.00008140255,0.0002345113,0.0000140497,0.7255969,0.0009622223,0.00006420164,0.2628095],"study_design_scores_gemma":[0.0153169,0.01291914,0.1547545,0.0008059305,0.0002969342,0.002048347,0.0005073801,0.001778975,0.7267445,0.0495679,0.03490432,0.000355197],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.4234386,0.003181166,0.5003294,0.02217232,0.0003711513,0.04976538,0.0001072598,0.00002985559,0.0006049184],"genre_scores_gemma":[0.2390774,0.0005872264,0.7424369,0.001042036,0.00133574,0.01478586,0.00002687968,0.00004938361,0.000658588],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.2624543,"threshold_uncertainty_score":0.3460256,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.1089613209462377,"score_gpt":0.4348466929901973,"score_spread":0.3258853720439596,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}