{"id":"W2095660210","doi":"10.2174/1874070700802010036","title":"Impact of Glycosylation on Saccharomyces cerevisiae Endopolygalacturonase PGU1 Activity and Stability","year":2008,"lang":"en","type":"article","venue":"The Open Biotechnology Journal","topic":"Polysaccharides and Plant Cell Walls","field":"Agricultural and Biological Sciences","cited_by":2,"is_retracted":false,"has_abstract":true,"ca_institutions":"Université du Québec à Trois-Rivières; Université Laval","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Glycosylation; Biochemistry; Saccharomyces cerevisiae; Enzyme; N-linked glycosylation; Chemistry; Mutagenesis; Recombinant DNA; Mutant; Protein engineering; Glycoprotein; Yeast; Gene; Glycan","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001650464,0.0003499595,0.0002088301,0.0001231819,0.00008024173,0.0003928126,0.0001544339,0.0001811099,0.0006432569],"category_scores_gemma":[0.0003305776,0.0001148015,0.0001906344,0.0002137067,0.0001729221,0.0001520406,0.0001851662,0.0003122768,0.0001976792],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0001982048,"about_ca_system_score_gemma":0.0001442775,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0009934272,"about_ca_topic_score_gemma":0.0007301074,"domain_scores_codex":[0.9998055,0.00004300011,0.00002276581,0.00003456052,0.00006388394,0.00003027883],"domain_scores_gemma":[0.9998223,0.00004719485,0.00005276813,0.00001680058,0.00002990857,0.00003094181],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002068909,0.00002753,0.001222407,0.00003531657,0.0000101577,0.00004949355,0.00002669338,0.0001649806,0.9965609,0.00003398169,0.0000221946,0.001639425],"study_design_scores_gemma":[0.000005416328,0.0002399517,0.0120831,0.000008534622,0.00002237845,0.0001880622,0.00005231307,0.0006370794,0.9858725,0.00002344751,0.0008587904,0.000008520398],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.998356,0.0006672299,0.0003301927,0.00002371941,0.000006574994,0.000003279323,0.0001603324,0.00001503869,0.0004374839],"genre_scores_gemma":[0.9986015,0.0002968758,0.000443969,0.00001301443,0.000001620375,0.000002774329,0.0002890849,0.00001128896,0.0003398798],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0009934272,"threshold_uncertainty_score":0.002151906,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.04213279848382952,"score_gpt":0.2628498898864975,"score_spread":0.220717091402668,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}