{"id":"W2095920248","doi":"10.1111/j.1462-2920.2004.00702.x","title":"Detection and quantification of the human‐specific HF183 <i>Bacteroides</i> 16S rRNA genetic marker with real‐time PCR for assessment of human faecal pollution in freshwater","year":2004,"lang":"en","type":"article","venue":"Environmental Microbiology","topic":"Fecal contamination and water quality","field":"Environmental Science","cited_by":358,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Saskatchewan","funders":"Vlaamse regering","keywords":"Biology; Amplicon; Bacteroides; 16S ribosomal RNA; Primer (cosmetics); Real-time polymerase chain reaction; Melting curve analysis; Polymerase chain reaction; Enumeration; Ribosomal RNA; SYBR Green I; Microbiology; Molecular biology; Bacteria; Genetics; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001279568,0.0009658401,0.000725267,0.0006237312,0.0001783635,0.0006827202,0.0003799693,0.0007606315,0.0006376997],"category_scores_gemma":[0.001449067,0.0004141461,0.0004577359,0.0006124626,0.0004725926,0.000373509,0.0004925442,0.0004729899,0.0004126874],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003186189,"about_ca_system_score_gemma":0.0004350668,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0007961718,"about_ca_topic_score_gemma":0.001325883,"domain_scores_codex":[0.9986388,0.000387861,0.0001090416,0.0002962398,0.0004421946,0.0001259262],"domain_scores_gemma":[0.9993529,0.0001758365,0.0002138899,0.00004289227,0.0001657719,0.00004880462],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0000804825,0.00002047587,0.002531898,0.0001392715,0.000008261323,0.00003201247,0.00004276493,0.000188381,0.991421,0.00005310298,0.0000424278,0.005439856],"study_design_scores_gemma":[0.0000247356,0.0009699506,0.024099,0.00008008743,0.00007301959,0.0004895373,0.0001202072,0.005475857,0.9639242,0.0001715377,0.004520112,0.00005187696],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.7213876,0.005322633,0.2683787,0.0001860659,0.0001280471,0.0004693656,0.001536868,0.0006135578,0.001977241],"genre_scores_gemma":[0.6280332,0.003465731,0.3619589,0.0002045989,0.00004828876,0.001116104,0.001792887,0.00008992263,0.003290503],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001279568,"threshold_uncertainty_score":0.006767035,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01260468657481495,"score_gpt":0.2305508728778027,"score_spread":0.2179461863029877,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}