{"id":"W2096039524","doi":"10.1371/journal.pone.0009731","title":"Assessing the Performance Capabilities of LRE-Based Assays for Absolute Quantitative Real-Time PCR","year":2010,"lang":"en","type":"article","venue":"PLoS ONE","topic":"Molecular Biology Techniques and Applications","field":"Biochemistry, Genetics and Molecular Biology","cited_by":71,"is_retracted":false,"has_abstract":true,"ca_institutions":"Canadian Forest Service; Natural Resources Canada","funders":"","keywords":"Amplicon; Biological system; Calibration; Physics; Statistics; Mathematics; Biology; Polymerase chain reaction; Genetics","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.01437011,0.001837183,0.001170051,0.001534522,0.0005629634,0.002878692,0.002335298,0.001953778,0.003158743],"category_scores_gemma":[0.0197666,0.0007146486,0.0009432404,0.001387195,0.00201517,0.00175976,0.00141672,0.002564395,0.003342321],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.001091307,"about_ca_system_score_gemma":0.0008701386,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0004914796,"about_ca_topic_score_gemma":0.0007944801,"domain_scores_codex":[0.9855204,0.004252756,0.001081681,0.002524642,0.005912351,0.0007081455],"domain_scores_gemma":[0.9853497,0.008950037,0.001704911,0.001635399,0.002162735,0.0001973052],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002873489,0.0002666205,0.005253867,0.001316284,0.00008414103,0.000136546,0.0004563424,0.007315235,0.9320691,0.004265687,0.001174761,0.04737406],"study_design_scores_gemma":[0.0000138125,0.0007708083,0.00347053,0.0001616249,0.00005997267,0.0003935199,0.0001351302,0.02596476,0.9571934,0.001976107,0.009754994,0.0001054603],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.133159,0.004402959,0.8501006,0.0006545442,0.000349098,0.0004906722,0.00134588,0.002412892,0.007084356],"genre_scores_gemma":[0.4762619,0.004268758,0.5067161,0.0006811405,0.0002107736,0.001924551,0.002506038,0.0006646541,0.006766037],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.01437011,"threshold_uncertainty_score":0.07599735,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03492981442867679,"score_gpt":0.2982229857943317,"score_spread":0.2632931713656549,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}