{"id":"W2096691349","doi":"10.1073/pnas.0700059104","title":"Structural basis for reversible photobleaching of a green fluorescent protein homologue","year":2007,"lang":"en","type":"article","venue":"Proceedings of the National Academy of Sciences","topic":"Advanced Fluorescence Microscopy Techniques","field":"Biochemistry, Genetics and Molecular Biology","cited_by":237,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Alberta","funders":"National Center for Research Resources; National Institute of General Medical Sciences","keywords":"Fluorescence; Photobleaching; Chromophore; Fluorescence recovery after photobleaching; Isomerization; Chemistry; Green fluorescent protein; Photochemistry; Fluorescence microscope; Fluorescent protein; Fluorescent light; Biophysics; Microscopy; Blue light; Catalysis; Materials science; Biology; Biochemistry; Optics","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001047624,0.00008047098,0.0001155947,0.00008840645,0.0001091971,0.00000454053,0.0005894691,0.0000986926,0.000001725288],"category_scores_gemma":[0.000455675,0.00005827448,0.00008153748,0.0002455263,0.0006245805,0.00002288165,0.0001418788,0.00008093417,4.198943e-8],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00002066798,"about_ca_system_score_gemma":0.00003032726,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00001739601,"about_ca_topic_score_gemma":4.356799e-7,"domain_scores_codex":[0.9990029,0.000003372382,0.0002708399,0.000208229,0.0003604718,0.0001542236],"domain_scores_gemma":[0.9992942,0.00002312453,0.0003758331,0.00001153767,0.0002738725,0.00002143283],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00004913667,0.00001308819,0.002582517,0.000078181,0.000008109289,1.809478e-9,0.00004529418,0.000007577151,0.9938876,0.002763366,0.0001251994,0.0004399437],"study_design_scores_gemma":[0.0001090433,0.0001298587,0.006114993,0.00009049423,0.00000531088,0.000003015229,0.00008707164,0.00006560543,0.985048,0.008170665,0.0001128386,0.00006309059],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9986203,0.0001135441,0.0002363279,0.0002801128,0.000008172347,0.000431498,0.00003373661,0.000005454733,0.0002708862],"genre_scores_gemma":[0.9208961,0.000007691325,0.07890259,0.00006532681,0.0000408768,0.00001097272,5.260937e-7,0.000004302819,0.00007163939],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.07866626,"threshold_uncertainty_score":0.2376364,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02590336057786113,"score_gpt":0.322018036328877,"score_spread":0.2961146757510159,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}