{"id":"W2096769332","doi":"10.1002/pro.2602","title":"Asymmetric mutations in the tetrameric R67 dihydrofolate reductase reveal high tolerance to active‐site substitutions","year":2014,"lang":"en","type":"article","venue":"Protein Science","topic":"Bacterial Genetics and Biotechnology","field":"Biochemistry, Genetics and Molecular Biology","cited_by":10,"is_retracted":false,"has_abstract":true,"ca_institutions":"Université de Montréal; PROTEO; Centre in Green Chemistry and Catalysis","funders":"Natural Sciences and Engineering Research Council of Canada; Concordia University; Deutscher Akademischer Austauschdienst","keywords":"Dihydrofolate reductase; Active site; Tetramer; Enzyme kinetics; Thermostability; Enzyme; Allosteric regulation; Stereochemistry; Biology; Biochemistry; Chemistry; Protein structure","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0005464502,0.0001138603,0.00009905334,0.00019401,0.0002498863,0.00006879676,0.0006238949,0.00008757144,0.000003819945],"category_scores_gemma":[0.0004764855,0.00008712626,0.00002965859,0.00181435,0.0004792539,0.000009931073,0.0001370153,0.0001326193,0.00002897042],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00002207846,"about_ca_system_score_gemma":0.000108944,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0001104863,"about_ca_topic_score_gemma":0.00006189947,"domain_scores_codex":[0.9987773,0.00007040698,0.0001692384,0.0004520464,0.0001904133,0.0003406185],"domain_scores_gemma":[0.999294,0.00001197912,0.00006186983,0.0004751649,0.00008382095,0.00007317932],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001759325,0.00004070196,0.0000913406,0.000002598603,0.000001477209,9.344845e-7,0.00005142172,0.000117067,0.9879014,0.0009520239,0.0000351337,0.01078825],"study_design_scores_gemma":[0.0002112141,0.0003848324,0.01946227,0.00001357034,0.00000476873,0.00001451099,0.0000298593,0.0001439391,0.9635889,0.0005848199,0.0153763,0.0001850384],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9950569,0.000072321,0.001946313,0.001768812,0.0001451534,0.0004877903,0.00002040214,0.00001177715,0.0004905544],"genre_scores_gemma":[0.9949128,0.00001448915,0.004397041,0.0003443233,0.0001040395,0.0001159717,0.00001607513,0.000006110464,0.00008917115],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.02431258,"threshold_uncertainty_score":0.3552906,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.00721370886581812,"score_gpt":0.2463950068971824,"score_spread":0.2391812980313643,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}