{"id":"W2096769332","doi":"10.1002/pro.2602","title":"Asymmetric mutations in the tetrameric R67 dihydrofolate reductase reveal high tolerance to active‐site substitutions","year":2014,"lang":"en","type":"article","venue":"Protein Science","topic":"Bacterial Genetics and Biotechnology","field":"Biochemistry, Genetics and Molecular Biology","cited_by":10,"is_retracted":false,"has_abstract":true,"ca_institutions":"Université de Montréal; PROTEO; Centre in Green Chemistry and Catalysis","funders":"Natural Sciences and Engineering Research Council of Canada; Concordia University; Deutscher Akademischer Austauschdienst","keywords":"Dihydrofolate reductase; Active site; Tetramer; Enzyme kinetics; Thermostability; Enzyme; Allosteric regulation; Stereochemistry; Biology; Biochemistry; Chemistry; Protein structure","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.00008151692,0.0002684923,0.0002195381,0.00019968,0.00008139341,0.0001994617,0.0002048172,0.0002378019,0.0005733884],"category_scores_gemma":[0.0001565723,0.0001122346,0.000171607,0.0001510684,0.000167434,0.00008703423,0.0001871814,0.0003548307,0.0002806976],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000199437,"about_ca_system_score_gemma":0.0001035728,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0004063493,"about_ca_topic_score_gemma":0.0004883352,"domain_scores_codex":[0.9998456,0.0000331993,0.00001606913,0.0000286613,0.00004452458,0.00003186334],"domain_scores_gemma":[0.9998685,0.00002575389,0.00004682643,0.00001838702,0.00001148679,0.00002909344],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003537428,0.00001178298,0.0001235778,0.000005584058,0.000002723992,0.0000308032,0.000005739467,0.00003445835,0.9991552,0.00004305335,0.00000702576,0.0005446756],"study_design_scores_gemma":[0.00001191894,0.0002397447,0.006372245,0.00000324451,0.00001843104,0.0005704451,0.0000251572,0.001513596,0.9904197,0.00004215939,0.0007767219,0.000006732411],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9979195,0.0001438581,0.001467787,0.0000150334,0.000005146545,0.000007515981,0.00008375799,0.00003399474,0.000323477],"genre_scores_gemma":[0.9981674,0.0001098411,0.001002061,0.00001572127,0.000002963807,0.000005564256,0.0001742264,0.00002315386,0.0004989877],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0005733884,"threshold_uncertainty_score":0.001918197,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.00721370886581812,"score_gpt":0.2463950068971824,"score_spread":0.2391812980313643,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}