{"id":"W2096868507","doi":"10.1002/bit.10774","title":"Large‐scale transient transfection of serum‐free suspension‐growing HEK293 EBNA1 cells: Peptone additives improve cell growth and transfection efficiency","year":2003,"lang":"en","type":"article","venue":"Biotechnology and Bioengineering","topic":"Viral Infectious Diseases and Gene Expression in Insects","field":"Biochemistry, Genetics and Molecular Biology","cited_by":132,"is_retracted":false,"has_abstract":true,"ca_institutions":"National Research Council Canada; Biotechnology Research Institute","funders":"","keywords":"Transfection; Green fluorescent protein; Cell culture; Molecular biology; Bioreactor; Recombinant DNA; HEK 293 cells; Chemistry; Secretory protein; Biology; Biochemistry; Secretion; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001168217,0.0002111231,0.0001990381,0.0001331693,0.0001481657,0.00001198288,0.0000787439,0.0004201542,0.0000124977],"category_scores_gemma":[0.00002332987,0.000200635,0.00009192702,0.0001648945,0.0001347085,0.0000153144,0.00003226282,0.0001632044,4.680506e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000006913564,"about_ca_system_score_gemma":0.00001698712,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00000834925,"about_ca_topic_score_gemma":0.000013799,"domain_scores_codex":[0.9989727,0.00003860489,0.0002146015,0.0004175013,0.00007537863,0.0002812044],"domain_scores_gemma":[0.9996309,0.00001290059,0.00004139064,0.0002040106,0.00003430219,0.0000765057],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003640876,0.0001159095,0.0001770327,0.0001672001,0.00003082041,0.000001836721,0.0001122575,0.000100283,0.9985119,0.0002332025,0.00002827858,0.0004849424],"study_design_scores_gemma":[0.001007178,0.0008072825,0.0004716023,0.00003832202,0.00004740246,0.00002926759,0.0003934055,0.0002919217,0.9958352,0.0000456571,0.0008026678,0.0002300235],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9739719,0.002275069,0.02285851,0.00007870473,0.0002775231,0.0002375149,0.00008987635,0.00006583243,0.0001450611],"genre_scores_gemma":[0.9977374,0.001732891,0.0003826114,0.00002944168,0.0000405399,0.00001881853,0.00001143119,0.0000215824,0.000025288],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.02376549,"threshold_uncertainty_score":0.8181658,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.002598245318232728,"score_gpt":0.1839236115129838,"score_spread":0.1813253661947511,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}