{"id":"W2096868507","doi":"10.1002/bit.10774","title":"Large‐scale transient transfection of serum‐free suspension‐growing HEK293 EBNA1 cells: Peptone additives improve cell growth and transfection efficiency","year":2003,"lang":"en","type":"article","venue":"Biotechnology and Bioengineering","topic":"Viral Infectious Diseases and Gene Expression in Insects","field":"Biochemistry, Genetics and Molecular Biology","cited_by":132,"is_retracted":false,"has_abstract":true,"ca_institutions":"National Research Council Canada; Biotechnology Research Institute","funders":"","keywords":"Transfection; Green fluorescent protein; Cell culture; Molecular biology; Bioreactor; Recombinant DNA; HEK 293 cells; Chemistry; Secretory protein; Biology; Biochemistry; Secretion; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0009684382,0.0005865764,0.001050232,0.0003128305,0.0002636897,0.0007280955,0.0007368592,0.0006294462,0.001666078],"category_scores_gemma":[0.0004196853,0.0002592475,0.0004683966,0.0006169251,0.0003493541,0.0004802567,0.0006112124,0.001330054,0.001394614],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004867168,"about_ca_system_score_gemma":0.000324559,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0005065215,"about_ca_topic_score_gemma":0.000694466,"domain_scores_codex":[0.9993912,0.0001063998,0.0001008148,0.0001265092,0.0001946266,0.00008037312],"domain_scores_gemma":[0.9997521,0.00008430429,0.00003451125,0.00004565789,0.00005541855,0.00002796054],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002860913,0.00002269028,0.00004470297,0.00005045006,0.000003859711,0.00002958027,0.000016715,0.00006324386,0.9987771,0.0000712878,0.00006708906,0.0008247595],"study_design_scores_gemma":[0.00001009565,0.00006827912,0.0003515191,0.000004659303,0.00001087177,0.00007921567,0.00001058127,0.001626205,0.9946577,0.00003017129,0.003145647,0.000005076869],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.7754512,0.004489425,0.2054718,0.0006052587,0.0003332921,0.0007974412,0.005384183,0.00178522,0.005682297],"genre_scores_gemma":[0.7587031,0.003785046,0.2086587,0.0003031913,0.00006393253,0.0008206536,0.01091421,0.0004816609,0.01626941],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001666078,"threshold_uncertainty_score":0.005573571,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.002598245318232728,"score_gpt":0.1839236115129838,"score_spread":0.1813253661947511,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}